14-3-3 antibody [3F7]
- Host / ClonalityMouse Monoclonal
- Clone Name3F7
- ApplicationsIP, WB
- Species reactivityHuman
14-3-3 antibody [3F7] validated data
14-3-3 antibody [3F7]
|Full Name||tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide|
|Product Description||Mouse monoclonal [3F7] to 14 - 3 - 3|
|Specificity||Recognizes human beta, gamma, sigma and zeta isoforms of 14-3-3|
|Background||Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf-1 kinase activity and phosphorylation amoung many other functions.|
|Immunogen||Recombinant full length protein (Human 14-3-3 gamma).|
Not tested in other applications.
|Western blot||1 - 2 ug/ml*
*Optimal dilutions/concentrations should be determined by the researcher.
|Positive Controls||HeLa whole cell lysate|
|Purification||Protein G purified|
|Storage Buffer||Preservative: 0.09% Sodium Azide; Constituents: 0.2% BSA, PBS. pH 7.4|
|Storage Instruction||Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.|
for 14-3-3 antibody [3F7]
Why is the observed Western Blot band size different from predicted size?
The predicted M.W. is based on protein sequence analysis; however, some factors might lead to an observed band size that is different from the predicted size. The reasons might include:
1.Post-translational modification (PTM):
a. Some post-translational modifications might lead to increased protein size, including
phosphorylation, acetylation, methylation, glycosylation, sumoylation, ubiquitination,
b. Some post-translational modifications might lead to decreased protein size including
phosphatidylethanolamine conjunction (e.g. LC3-II)
c. Some proteins may be cleaved to form an active or mature form; this process will
lead to a decreased protein size (e.g. Notch activation, Caspase activation, etc.)
d. Some websites provide useful PTM information
iv.CBS data sets http://www.cbs.dtu.dk/databases/
v.CBS prediction Servers http://www.cbs.dtu.dk/services/
2.mRNA splice variants (Isoforms):
Through alternative splicing, one gene can generate different proteins with different M.W. Regulation of alternative splicing depends upon cell type, conditions, etc.
Some proteins could form dimers or multimers, increasing the M.W. This phenomenon usually can be found in reducing gel condition; however, strong interactions may still be seen with higher molecular weight proteins even in denaturing gel.
The observed size could also potentially be influenced by the protein charge
Different species likely have different protein sequence and PTM, which can lead to a different protein M.W.