| Catalog Number | GTX14108 |
| Product Name | 14-3-3 antibody [3F7]
see all '14-3-3' products |
| Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide |
| Synonyms | | 1433 | |
| Product Description | Mouse monoclonal [3F7] to 14 - 3 - 3 |
| Background | Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf-1 kinase activity and phosphorylation amoung many other functions. |
| Specificity | Recognizes human beta, gamma, sigma and zeta isoforms of 14-3-3 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No | 3F7 |
| Subclass | IgG1 |
| Immunogen | Recombinant full length protein (Human 14-3-3 gamma). |
| Antigen Species | Human |
| Species Reactivity | Human |
| Applications | Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 30 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Positive Controls | HeLa whole cell lysate |
| Target | 14-3-3 |
| Cellular Localization | Cytoplasmic |
| Form Supplied | Liquid |
| Concentration | 0.1 mg/ml |
| Purification | Protein G purified |
| Storage Buffer | Preservative: 0.09% Sodium Azide; Constituents: 0.2% BSA, PBS. pH 7.4 |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |