| Catalog Number | GTX61021 |
| Product Name | 14-3-3 b/a antibody [E58], N-term
see all '14-3-3 alpha ' products |
| Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide |
| Synonyms | | KCIP-1 | KCIP1 | YWHAB | HS1 | GW128 | YWHAA | KCIP 1 | |
| Product Description | Rabbit monoclonal antibody [E58] to 14-3-3 b/a (N-term) |
| Background | The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints . The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins . Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol |
| Specificity | Predicted to cross-react with sheep and bovine, based on sequence homology. |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No | E58 |
| Subclass | IgG |
| Immunogen | A synthetic peptide corresponding to residues near N-terminus of human 14-3-3 beta/alpha was used as immunogen. |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse, Rat |
| Cross Reactivity Note | GTX61021 was generated against human protein. Species cross-reactivity is determined by Western Blot. Crossreactivity with other species cannot be guaranteed for other applications. |
| Applications | Flow cytometry/FACS, IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | Recommended Starting Dilutions:
For WB: Use at a dilution of 1:1,000
For IHC: Use at a dilution of 1:250
For FACS: Use at a dilution of 1:50
Optimal working dilution for a specific application should be determined by the investigator. |
| Target | 14-3-3 b/a (N-term) |
| Predicted Target Size(KDa) | 30 (Note) |
| Form Supplied | Liquid |
| Purification | Cell Supernatant |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage Instruction | Store at -20 °C. Stable for 12 months from date of receipt. |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. Product intellectual property information |