14-3-3 beta antibody [N1C2]
|Full Name||tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide|
|Synonyms||GW128 antibody, HS1 antibody, KCIP-1 antibody, YWHAA antibody, YWHAB antibody, "brain protein 14-3-3, beta isoform antibody", protein 1054 antibody, 14-3-3 protein beta/alpha antibody, protein kinase C inhibitor protein-1 antibody, "tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide antibody", 14-3-3 alpha antibody, protein kinase C inhibitor protein 1 antibody, "tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide antibody"|
|Product Description||Rabbit Polyclonal antibody to 14-3-3 beta (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide)|
|Background||This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq]|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 171 of 14-3-3 beta (Uniprot ID#P31946)|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rhesus Monkey, Bovine|
|Predict Reactivity Note||Rhesus Monkey (100%), Bovine (100%)|
|Applications||ICC/IF, IHC-P, WB|
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
IHC (Formalin-fixed paraffin-embedded sections) : suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain|
|Predicted Target Size||28 kDa (note)|
|Cellular Localization||Cytoplasm , Melanosome |
|Purification||Purified by antigen-affinity chromatography.|
|Storage Buffer||0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Storage Instruction||Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.|
|Notes||For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |