| Catalog Number | GTX113296 |
| Product Name | 14-3-3 beta antibody [N1C2]
see all '14-3-3 beta' products |
| Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide |
| Synonyms | GW128 antibody | HS1 antibody | KCIP-1 antibody | YWHAA antibody | YWHAB antibody | "brain protein 14-3-3, beta isoform antibody" | protein 1054 antibody | 14-3-3 protein beta/alpha antibody | protein kinase C inhibitor protein-1 antibody | "tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide antibody" | 14-3-3 alpha antibody | protein kinase C inhibitor protein 1 antibody | "tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide antibody" |
| Product Description | Rabbit Polyclonal antibody to 14-3-3 beta (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide) |
| Background | This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq] |
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1 and 171 of 14-3-3 beta (Uniprot ID#P31946) |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse |
| Predicted Cross Reactivity Species | Chicken (97%), Rat (97%), Bovine (100%), Xenopus tropicalis (87%) (Note) |
| Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Positive Controls | 293T | A431 | H1299 | HeLa | HepG2 | Molt-4 | Raji | mouse brain |
| Predicted Target Size(KDa) | 28 (Note) |
| Cellular Localization | Cytoplasm | Melanosome | |
| Form Supplied | Liquid |
| Concentration | 1 mg/ml |
| Purification | Purified by antigen-affinity chromatography. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Storage Instruction | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |