| Catalog Number | GTX12347 |
| Product Name | 14-3-3 beta + zeta antibody [22-IID8B]
see all '14-3-3 beta ' products |
| Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide |
| Synonyms | | YWHAA | KCIP1 | 1433 beta + zeta | HS1 | GW128 | 14-3-3-zeta | KCIP-1 | MGC111427 | MGC126532 | MGC138156 | YWHAD | KCIP 1 | 14 3 3 zeta | 1433zeta | |
| Product Description | Mouse monoclonal [22 - IID8B] to 14 - 3 - 3 beta + zeta |
| Background | 14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exist in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No | 22-IID8B |
| Subclass | IgG |
| Immunogen | Lysed transformed amnion cells (AMA) (Human). |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse, Cow, Dog, Monkey, Pig, Rabbit, Rat, Sheep |
| Applications | ICC/IF, Immunohistochemistry, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | ICC: Use at an assay dependent dilution. IHC: Use at a dilution of 1/50. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 29 and 30 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Positive Controls | HeLa cell lysate |
| Target | 14-3-3 beta + zeta |
| Cellular Localization | Cytoplasmic |
| Purification | Protein G purified |
| Storage Buffer | Preservative: NoneConstituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2 |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Notes | Storage below -20?C is not recommended.For maximum product recovery centrifuge the product vial before removing cap. |