14-3-3 epsilon antibody [N2C3]
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||29 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide|
|Product Description||Rabbit Polyclonal antibody to 14-3-3 epsilon (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide)|
|Background||This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq]|
|Synonyms||14-3-3E antibody, FLJ45465 antibody, FLJ53559 antibody, KCIP-1 antibody, MDCR antibody, MDS antibody, YWHAE antibody, "tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide antibody", protein kinase C inhibitor protein-1 antibody, mitochondrial import stimulation factor L subunit antibody, 14-3-3 epsilon antibody, 14-3-3 protein epsilon antibody, "tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide antibody"|
|Cellular Localization||Cytoplasm , Melanosome |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human 14-3-3 epsilon. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Xenopus laevis, Chicken, Chimpanzee, Bovine|
|Predict Reactivity Note||Rat (98%), Xenopus laevis (97%), Chicken (100%), Chimpanzee (100%), Bovine (100%)|