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Catalog Number
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GTX113298
(
1
)
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Product Name
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14-3-3 gamma antibody
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| Applications | ICC/IF, IHC-P, IP, WB |
| Application Note |
| Suggested dilution | Reference |
| Immunocytochemistry/ Immunofluorescence | 1:100-1:1000* |
|
| IHC (Formalin-fixed paraffin-embedded sections) | 1:100-1:1000* |
|
| Immunoprecipitation | Assay-dependent dilution |
Y |
| Western blot | 1:500-1:10000* |
|
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|
| Predicted Target Size | 28 kDa (note)
 (2)
|
| Positive Controls | K562 , THP-1 , HL-60 , 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain |
| Form Supplied | Liquid |
| Concentration | 1.03
mg/ml
(Please refer to the vial label for the specific concentration) |
| Purification | Purified by antigen-affinity chromatography. |
| Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide |
| Product Description | Rabbit Polyclonal antibody to 14-3-3 gamma (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide) |
| Background | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq] |
| Synonyms | 14-3-3GAMMA antibody, YWHAG antibody, KCIP-1 antibody, 14-3-3 gamma antibody, 14-3-3 protein gamma antibody, protein kinase C inhibitor protein 1 antibody, "tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide antibody" |
| Cellular Localization | Cytoplasm |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human 14-3-3 gamma. The exact sequence is proprietary. |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse |
Predicted Cross Reactivity species  Predicted cross-reactivity:
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
| Rat, Xenopus laevis, Chicken, Rhesus Monkey, Bovine |
| Predict Reactivity Note | Rat (100%), Xenopus laevis (98%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%) |
| Conjugation | Unconjugated |