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1 .GTX113298 14-3-3 gamma antibody

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14-3-3 gamma antibody

Comparative Abs

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, IP, WB
  • Species reactivityHuman, Mouse
  • Publication  ( 1 )
Cat No: GTX113298
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-14-3-3 gamma antibody used in Western Blot (WB). GTX113298
  • Anti-14-3-3 gamma antibody used in Western Blot (WB). GTX113298
  • Anti-14-3-3 gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113298
  • Anti-14-3-3 gamma antibody used in Western Blot (WB). GTX113298
  • Anti-14-3-3 gamma antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX113298
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  • Bio-info
  • Protocol & MSDS
  • Application Information: 14-3-3 gamma antibody

Catalog Number GTX113298
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( 0 )
Publication ( 1 )
Product Name 14-3-3 gamma antibody
See all 14-3-3 gamma products
ApplicationsICC/IF, IHC-P, IP, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
ImmunoprecipitationAssay-dependent dilution Y
Western Blot (WB)1:500-1:10000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size28 kDa (note) IsoForm (2)
Positive ControlsK562 , THP-1 , HL-60 , 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain
Form SuppliedLiquid
Concentration1.03 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: 14-3-3 gamma antibody

Full Nametyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
BackgroundThis gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq]
Synonyms14-3-3GAMMA antibody, YWHAG antibody, KCIP-1 antibody, 14-3-3 gamma antibody, 14-3-3 protein gamma antibody, protein kinase C inhibitor protein 1 antibody, "tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide antibody"
Cellular LocalizationCytoplasm
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human 14-3-3 gamma. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Xenopus laevis, Chicken, Rhesus Monkey, Bovine
Predict Reactivity NoteRat (100%), Xenopus laevis (98%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%)
ConjugationUnconjugated

  • Storage Conditions: 14-3-3 gamma antibody

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cell Biology > Protein Trafficking
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14-3-3 gamma antibody validated data
  • Anti-14-3-3 gamma antibody used in Western Blot (WB). GTX113298
    • GTX113298 WB Image
      Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 gamma antibody (GTX113298) diluted at 1:1000 and competitor's antibody (SC-731) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      *The competitor is not affiliated with GeneTex and does not endorse this product.
      Top
  • Anti-14-3-3 gamma antibody used in Western Blot (WB). GTX113298
    • GTX113298 WB Image
      Sample (50 µg of whole cell lysate)
      A: mouse brain
      12% SDS PAGE
      GTX113298 diluted at 1:10000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-14-3-3 gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113298
    • GTX113298 ICC/IF Image
      Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 gamma(GTX113298) antibody at 1:200 dilution.
      Top
  • Anti-14-3-3 gamma antibody used in Western Blot (WB). GTX113298
    • GTX113298 WB Image
      Sample (30 µg of whole cell lysate)
      A: 293T
      B: A431 (GTX27909)
      12% SDS PAGE
      GTX113298 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-14-3-3 gamma antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX113298
    • GTX113298 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using 14-3-3 gamma(GTX113298) antibody at 1:500 dilution.
      Top
  • Anti-14-3-3 gamma antibody used in Western Blot (WB). GTX113298
    • GTX113298 WB Image
      Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 gamma antibody (GTX113298) diluted at 1:1000 and competitor's antibody (SC-731) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      *The competitor is not affiliated with GeneTex and does not endorse this product.
      Top
  • Anti-14-3-3 gamma antibody used in Western Blot (WB). GTX113298
    • GTX113298 WB Image
      Sample (50 µg of whole cell lysate)
      A: mouse brain
      12% SDS PAGE
      GTX113298 diluted at 1:10000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-14-3-3 gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113298
    • GTX113298 ICC/IF Image
      Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 gamma(GTX113298) antibody at 1:200 dilution.
      Top
  • Anti-14-3-3 gamma antibody used in Western Blot (WB). GTX113298
    • GTX113298 WB Image
      Sample (30 µg of whole cell lysate)
      A: 293T
      B: A431 (GTX27909)
      12% SDS PAGE
      GTX113298 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-14-3-3 gamma antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX113298
    • GTX113298 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using 14-3-3 gamma(GTX113298) antibody at 1:500 dilution.
      Top
Back to Top
  • Reference for 14-3-3 gamma antibody
Application References Application Reactivity
Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell 61 (6):903-13 (2016). IP Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensYWHAG7532P61981605356BC020963AAH2096328.31
Homo sapiensYWHAG7532P61981605356NM_012479NP_03661128.31

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusYwhag22628P61982, A8IP69NM_018871NP_06135928.31
Rattus norvegicusYwhag56010P61983NM_019376NP_06224928.31
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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