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Catalog Number
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GTX113298
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Product Name
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14-3-3 gamma antibody
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| Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide |
| Synonyms | 14-3-3GAMMA antibody, YWHAG antibody, KCIP-1 antibody, 14-3-3 gamma antibody, 14-3-3 protein gamma antibody, protein kinase C inhibitor protein 1 antibody, "tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide antibody" |
| Product Description | Rabbit Polyclonal antibody to 14-3-3 gamma (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide) |
| Background | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq] |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 179 (P61981) of 14-3-3 gamma |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse |
| Predicted Cross Reactivity species | Rat, Rhesus Monkey, Bovine |
| Predict Reactivity Note | Rat (100%), Rhesus Monkey (100%), Bovine (100%) |
| Applications | ICC/IF, IHC-P, WB |
| Application Note |
| Suggested dilution | Protocol | Reference |
| ICC/IF | 1:100-1:1000* |
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| IHC (Formalin-fixed paraffin-embedded sections) | 1:100-1:1000* |
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| Western blot | 1:1000-1:10000* |
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IHC (Formalin-fixed paraffin-embedded sections) : suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
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| Positive Controls | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain |
| Predicted Target Size | 28 kDa |
| Cellular Localization | Cytoplasm |
| Form Supplied | Liquid |
| Purification | Purified by antigen-affinity chromatography. |
| Concentration | 1.03
mg/ml
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| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Storage Instruction | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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