14-3-3 (phospho Ser58) antibody
|Full Name||tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide|
|Synonyms||14-3-3 (phospho S58), 14-3-3 (phospho Ser58), 14-3-3 (pS58), 14-3-3 (pSer58), 14-3-3 phospho S58, 14-3-3 phospho Ser58, Phospho 14-3-3 (S58), Phospho 14-3-3 (Ser58), Phospho 14-3-3 (pS58), Phospho 14-3-3 (pSer58)|
|Product Description||Rabbit Polyclonal antibody to 14-3-3 (Phospho S58)|
|Specificity||GTX42911 specifically recognises the 29kDa 14-3-3 protein, when phosphorylated at Ser58. |
The family of 14-3-3 proteins are highly conserved proteins expressed abundantly in the brain, which have been identified as crucial regulators of many cellular processes including multiple signalling pathways, cell growth, apoptosis, intracellular trafficking and malignant transformation. The interaction of 14-3-3 with target proteins is typically dependant upon phosphorylation, occurring through phosphoserine and phosphothreonine binding, with 14-3-3 protein activity itself being regulated by kinases such as kinase A and C, Akt, and CaMK1 and equally by phosphatases such as protein phosphatase 1 (PP1) and 2A (PP2A).
The ability of 14-3-3 protein to facilitate the phosphorylation of Tau present in neurofibrillary tangles (NFTs) and the co-localisation of 14-3-3 protein with alpha-synuclein found in Lewy bodies, suggests a role for 14-3-3 protein in the pathogenesis of both Alzheimer’s disease and Parkinson’s disease (1).
|Target||14-3-3 (Phospho S58)|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within 14-3-3 protein which includes phosphorylated Ser58.|
|Application Note||Recommended Starting Dilutions:|
For WB: Use at 1/1000
Optimal dilutions should be determined experimentally by the researcher.
|Storage Buffer||10mM HEPES pH7.5, 0.09%Sodium Azide0.01%Bovine Serum Albumin50%Glycerol|
|Storage Instruction||Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.|
|Notes||For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |