Find Antibodies, Antibody Supplier - GeneTex Inc.
United States
  • Please select
    • All categories
    • Primary antibodies
    • Secondary antibodies
    • Antibody panels
    • Small molecules
    • Proteins
    • Peptides
    • Lysates
    • Slides
    • Sera & plasma
    • Research reagents
    • Research kits
    • Assay kits
    • ELISA kits
    • ELISA pairs
    • Isotype controls
My Account My Cart
Please login Product Support| Company News| Contact Us| SiteMap
  • Primary Antibodies

  • Secondary Antibodies

  • Proteins & Peptides

  • Lysates & Slides

  • Serum & Reagents

  • Research Kits

  • Isotype Controls

  • Home
  • Primary Antibodies
  • Primary antibodies
  • 4E-BP1 antibody [N1C3]

Related products
More Hide
Recently viewed products

1 .GTX109162 4E-BP1 antibody [N1C3]

GTX101435 beta Catenin antibody [N1N2-2], N-term
Anti-beta Catenin antibody [N1N2-2], N-term used in IHC-P. GTX101435
GTX122148 Histone H3 antibody
Anti-Histone H3 antibody used in IHC-P. GTX122148
GTX128508 mCherry antibody
Anti-mCherry antibody used in IHC-Wm. GTX128508
GTX629448 5-Methylcytosine / 5-mC antibody [GT4111]
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in Dot. GTX629448
GTX629765 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in MeDIP. GTX629765
GTX629890 SQSTM1 / P62 antibody [GT1478]
Anti-SQSTM1 / P62 antibody [GT1478] used in WB. GTX629890
ECL kit RIPA Extraction Kit Protein Ladder

4E-BP1 antibody [N1C3]

Knockout/Knockdown

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIHC-P, IP, WB
  • Species reactivityHuman, Rat
  • ★★★★★   (  1  ) Publication  ( 1 )
Cat No: GTX109162
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-4E-BP1 antibody [N1C3] used in Immunoprecipitation. GTX109162
  • Anti-4E-BP1 antibody [N1C3] used in Western Blot (WB). GTX109162
  • Anti-4E-BP1 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX109162
  • Anti-4E-BP1 antibody [N1C3] used in Western Blot (WB). GTX109162
LinkButton
  • Datasheet
  • Star Review (1)
    Reference (1)
  • Bio-info
  • Protocol & MSDS
  • Application Information: 4E-BP1 antibody [N1C3]

Catalog Number GTX109162
★★★★★
( 1 )
Publication ( 1 )
Product Name 4E-BP1 antibody [N1C3]
See all 4E-BP1 products
ApplicationsIHC-P, IP, WB
Application Note
Suggested dilutionReference
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Immunoprecipitation1:100-1:500*  
Western Blot (WB)1:500-1:3000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size13 kDa (note)
Positive ControlsHeLa , H1299 , HCT116 , *NB4 , *HL60
Form SuppliedLiquid
Concentration0.13 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: 4E-BP1 antibody [N1C3]

Full Nameeukaryotic translation initiation factor 4E binding protein 1
BackgroundThis gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq]
Synonyms4E-BP1 antibody, 4EBP1 antibody, BP-1 antibody, MGC4316 antibody, PHAS-I antibody, EIF4EBP1 antibody, eukaryotic translation initiation factor 4E-binding protein 1 antibody, eIF4E-binding protein 1 antibody, phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody, eukaryotic translation initiation factor 4E binding protein 1 antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenFull length human 4E-BP1 Recombinant protein.
Antigen SpeciesHuman
Species ReactivityHuman, Rat
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Mouse, Pig, Bovine
Predict Reactivity NoteMouse (90%), Pig (91%), Bovine (92%)
ConjugationUnconjugated

  • Storage Conditions: 4E-BP1 antibody [N1C3]

Storage Buffer1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Disease Related > Alzheimer's disease
  • Neuroscience > Disorder > Alzheimer's disease
More Hide
4E-BP1 antibody [N1C3] validated data
  • Anti-4E-BP1 antibody [N1C3] used in Immunoprecipitation. GTX109162
    • GTX109162 IP Image
      Immunoprecipitation of 4E-BP1 protein from HCT116 whole cell extracts using 5 μg of 4E-BP1 antibody [N1C3] (GTX109162) or 4E-BP1 antibody [N1C3-2] (GTX116315).
      Western blot analysis was performed using 4E-BP1 antibody [N1C3] (GTX109162).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-4E-BP1 antibody [N1C3] used in Western Blot (WB). GTX109162
    • GTX109162 WB Image
      Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3] (GTX109162) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      CRISPR knockout cell lines generated by www.edigene.com
      Top
  • Anti-4E-BP1 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX109162
    • GTX109162 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using EIF4EBP1(GTX109162) antibody at 1:500 dilution.
      Top
  • Anti-4E-BP1 antibody [N1C3] used in Western Blot (WB). GTX109162
    • GTX109162 WB Image
      4E-BP1 antibody detects 4E-BP1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody (GTX109162) diluted at a dilution of 1:2000.
      Top
  • Anti-4E-BP1 antibody [N1C3] used in Immunoprecipitation. GTX109162
    • GTX109162 IP Image
      Immunoprecipitation of 4E-BP1 protein from HCT116 whole cell extracts using 5 μg of 4E-BP1 antibody [N1C3] (GTX109162) or 4E-BP1 antibody [N1C3-2] (GTX116315).
      Western blot analysis was performed using 4E-BP1 antibody [N1C3] (GTX109162).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-4E-BP1 antibody [N1C3] used in Western Blot (WB). GTX109162
    • GTX109162 WB Image
      Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3] (GTX109162) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      CRISPR knockout cell lines generated by www.edigene.com
      Top
  • Anti-4E-BP1 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX109162
    • GTX109162 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using EIF4EBP1(GTX109162) antibody at 1:500 dilution.
      Top
  • Anti-4E-BP1 antibody [N1C3] used in Western Blot (WB). GTX109162
    • GTX109162 WB Image
      4E-BP1 antibody detects 4E-BP1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody (GTX109162) diluted at a dilution of 1:2000.
      Top
  • StarReview
  • 4E-BP1 antibody [N1C3]

  • Rating : ★★★★★ ( Average 5.0 based on 1 users reviews)
  • Western Blot (WB)(1) :★★★★★
  • Anonymous StarReview submitted on 07 Apr 2015
  • Score : ★★★★★
  • Application Tested : WB
  • Sample Species : Human
  • Sample : NB4 cells, HL60 cells
  • Amount used(ug/cells) : 20ug
  • Blocking conditions
  • Blocking Buffer : 5% non-fat milk
  • Incubation Time & Temperature : RT (℃)/1.5hr
  • Primary antibody
  • Dilution : 1:1000( conc. of dilution )
  • Incubation Time & Temperature : 4 (℃)/overnight
  • Data Description
  • Lane1: NB4 cell lysate. Lane2: HL60 cell lysate
Back to Top
  • Reference for 4E-BP1 antibody [N1C3]
Application References Application Reactivity
Yeh YH et al. Inflammatory interferon activates HIF-1α-mediated epithelial-to-mesenchymal transition via PI3K/AKT/mTOR pathway. J Exp Clin Cancer Res 37 (1):70 (2018). WB Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensEIF4EBP11978Q13541, B2R502602223NM_004095NP_00408612.58

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusEif4ebp113685Q60876NM_007918NP_03194412.33
Rattus norvegicusEif4ebp1116636Q62622NM_053857NP_44630912.41
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-ProClin.pdf
  • Product types

    Primary Antibodies Secondary Antibodies Proteins & Peptides Lysates & Slides Serum & Reagents Research Kits Isotype Controls

    Resources

    Literature Newsletters Protocols Pathways

  • Research Areas

    Cancer Cell Biology Neuroscience Metabolism Epigenetics Immunology Signal Transduction Infectious Disease Disease Related Stem Cell Development Zebrafish Epitope Tags

  • Company

    About Us News Rewards Program Recent Publications Contact Us Policy Guarantee Careers Sitemap

    Support

    Protocols Technical Tips FAQ

  • Distributors

    Custom Antibodies

    Member Account

    • Youtube Youtube
    • Facebook Facebook
    • Twitter Twitter
    • LinkedIn LinkedIn
    • Weibo Weibo
© 2013 GeneTex Inc. All rights reserved.
All products are for research use only—Not for use in diagnostic procedures.