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  • 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]

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1 .GTX629765 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]

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GTX629765 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]
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5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]


  • Host / ClonalityMouse Monoclonal
  • Clone NameGT13612
  • ApplicationsDot, IHC-Fr, IHC-P, MeDIP
  • Publication  ( 1 )
Cat No: GTX629765
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Methylated DNA Immunoprecipitation. GTX629765
  • Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Formalin-fixed paraffin-embedded sections). GTX629765
  • Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Formalin-fixed paraffin-embedded sections). GTX629765
  • Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Formalin-fixed paraffin-embedded sections). GTX629765
  • Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Dot blot. GTX629765
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  • Datasheet
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  • Protocol & MSDS

  • Application Information: 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]

Catalog Number GTX629765
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Publication ( 1 )
Product Name 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]
See all 5-Hydroxymethylcytosine / 5-hmC products
ApplicationsDot, IHC-Fr, IHC-P, MeDIP
Application Note
Suggested dilutionReference
Dot blotAssay-dependent dilution  
IHC (Frozen sections)Assay-dependent dilution  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Methylated DNA ImmunoprecipitationAssay-dependent dilution  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationAffinity purified by Protein G.

  • Specifications: 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]

Product DescriptionMouse monoclonal antibody [GT13612] to 5-Hydroxymethylcytosine / 5-hmC
Synonyms5hmc Antibody, 5-hmc Antibody, 5-HydroxymethylCytidine Antibody
HostMouse
ClonalityMonoclonal
Clone NameGT13612
IsotypeIgG2b
ConjugationUnconjugated

  • Storage Conditions: 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]

Storage BufferPBS
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] validated data
  • Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Methylated DNA Immunoprecipitation. GTX629765
    • GTX629765 MeDIP Image
      GTX629765 5-HydroxymethylCytidine antibody [GT13612] in MeDIP experiment.
      Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-HydroxymethylCytidine antibody [GT13612] (GTX629765) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.
      Top
  • Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Formalin-fixed paraffin-embedded sections). GTX629765
    • GTX629765 IHC-P Image
      5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse fore brain by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse fore brain.
      5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.
      Top
  • Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Formalin-fixed paraffin-embedded sections). GTX629765
    • GTX629765 IHC-P Image
      5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse prostate by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse prostate.
      5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.
      Top
  • Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Formalin-fixed paraffin-embedded sections). GTX629765
    • GTX629765 IHC-P Image
      5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse testis by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse testis.
      5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.
      Top
  • Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Dot blot. GTX629765
    • GTX629765 Dot Image
      GTX629765 5-hydroxymethylcytosine antibody [GT13612] Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX629765) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine
      Top
  • Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Methylated DNA Immunoprecipitation. GTX629765
    • GTX629765 MeDIP Image
      GTX629765 5-HydroxymethylCytidine antibody [GT13612] in MeDIP experiment.
      Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-HydroxymethylCytidine antibody [GT13612] (GTX629765) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.
      Top
  • Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Formalin-fixed paraffin-embedded sections). GTX629765
    • GTX629765 IHC-P Image
      5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse fore brain by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse fore brain.
      5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.
      Top
  • Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Formalin-fixed paraffin-embedded sections). GTX629765
    • GTX629765 IHC-P Image
      5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse prostate by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse prostate.
      5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.
      Top
  • Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Formalin-fixed paraffin-embedded sections). GTX629765
    • GTX629765 IHC-P Image
      5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse testis by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse testis.
      5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.
      Top
  • Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Dot blot. GTX629765
    • GTX629765 Dot Image
      GTX629765 5-hydroxymethylcytosine antibody [GT13612] Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX629765) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine
      Top
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  • Reference for 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]
Application References Application Reactivity
Hsieh MC et al. Tet1-dependent epigenetic modification of BDNF expression in dorsal horn neurons mediates neuropathic pain in rats. Sci Rep 6:37411 (2016). IHC-Fr, Dot
    *MSDS
    MSDS-PBS.pdf

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