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5-Methylcytosine / 5-mC antibody [33D3]

Cat. No. GTX75897

Host

Mouse

Clonality

Monoclonal

Clone Name

33D3

Isotype

IgG1

Application

ICC/IF, IHC-P, IHC-Fr, FACS, ELISA, Immunoblotting, MeDIP

Reactivity

Species independent
Package
50 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
ELISA Assay dependent
Immunoblotting Assay dependent
MeDIP Assay dependent
Not tested in other applications.

Product Note

This antibody recognizes 5-Methylcytosine. Clone 33D3 is specific to the methylated base and has minimal reactivity to non-methylated cytidine or cytosine (Reynaudet al.1992).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_10728896

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

REFERENCE

REVIEW

5-Methylcytosine / 5-mC antibody [33D3] Cat. No. GTX75897

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2013. PMID: 23562889
SDS
PBS.pdf
Package List Price ($)
$ 399