5-Methylcytosine / 5-mC antibody [GT4111]

Host / ClonalityMouse Monoclonal
Clone NameGT4111
ApplicationsDot, EM, IHC-P, IHC-Wm, MeDIP
★★★★★ ( 2 ) ( 4 )

Cat No:

GTX629448

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

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Datasheet
Star Review (2)
Reference (4)
Protocol & MSDS

Application Information: 5-Methylcytosine / 5-mC antibody [GT4111]

Catalog Number

GTX629448

Product Name

5-Methylcytosine / 5-mC antibody [GT4111]

See all 5-Methylcytosine / 5-mC products

Applications

Dot, EM, IHC-P, IHC-Wm, MeDIP

Application Note

	Suggested dilution	Reference
Dot blot	Assay-dependent dilution	Y
Electron microscopy	Assay-dependent dilution	Y
IHC (Formalin-fixed paraffin-embedded sections)	1:100-1:1000*	Y
IHC (Whole mount)	1:50-1:500*
Methylated DNA Immunoprecipitation	Assay-dependent dilution

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Positive Controls

GTX400004 , *SH-SY5Y genomic DNA

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Affinity purified by Protein G.

Specifications: 5-Methylcytosine / 5-mC antibody [GT4111]

Product Description	Mouse Monoclonal antibody [GT4111] to 5-Methylcytosine / 5-mC
Host	Mouse
Clonality	Monoclonal
Clone Name	GT4111
Target	5-Methylcytosine
Isotype	IgG1
Conjugation	Unconjugated

Storage Conditions: 5-Methylcytosine / 5-mC antibody [GT4111]

Storage Buffer	PBS
Storage Instruction	Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

5-Methylcytosine / 5-mC antibody [GT4111] validated data

GTX629448 MeDIP Image

GTX629448 5-Methylcytosine/ 5-mC antibody [GT4111] in MeDIP experiment.
Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Methylcytosine/ 5-mC antibody [GT4111] (GTX629448) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

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GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

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GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

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GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

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GTX629448 Dot Image

GTX629448 5-methylcytosine antibody [GT4111] Dot blot analysis of anti-5-mC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody (GTX629448) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine

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GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleolus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) diluted at 1:200.

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GTX629448 IHC-Wm Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 24 hours-post-fertilization zebrafish embryo.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:50.

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GTX629448 IHC-Wm Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 24 hours-post-fertilization zebrafish embryo.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:50.

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GTX629448 MeDIP Image

GTX629448 5-Methylcytosine/ 5-mC antibody [GT4111] in MeDIP experiment.
Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Methylcytosine/ 5-mC antibody [GT4111] (GTX629448) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

Top

GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

Top

GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

Top

GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

Top

GTX629448 Dot Image

GTX629448 5-methylcytosine antibody [GT4111] Dot blot analysis of anti-5-mC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody (GTX629448) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine

Top

GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleolus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) diluted at 1:200.

Top

GTX629448 IHC-Wm Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 24 hours-post-fertilization zebrafish embryo.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:50.

Top

GTX629448 IHC-Wm Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 24 hours-post-fertilization zebrafish embryo.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:50.

Top

StarReview
5-Methylcytosine / 5-mC antibody [GT4111]
Rating : ★★★★★ ( Average 5.0 based on 2 users reviews)
Dot blot(1) :★★★★☆
IHC (Formalin-fixed paraffin-embedded sections)(1) :★★★★★

Anonymous StarReview submitted on 07 Mar 2014
Score : ★★★★☆
Application Tested : Dot blot
Sample : SH-SY5Y genomic DNA
Blocking conditions
Blocking Buffer : 5% milk in PBST (1x PBS + 0,05% Tween-20)
Incubation Time & Temperature : 25 (℃)/1hr
Primary antibody
Dilution : 1:500( conc. of dilution )
Incubation Time & Temperature : 25 (℃)/1hr

Anonymous StarReview submitted on 15 Dec 2015
Score : ★★★★★
Application Tested : IHC
Sample : endometrium tissue
Permeabilization/Antigen retrieval
Permeabilization/Antigen retrieval Buffer : 0.2 % Triton X-100 in PBS/ citrate buffer (10 mM, pH 6.0)
Blocking conditions
Blocking Buffer : commercial blocking buffer
Incubation Time & Temperature : RT (℃)/1hr
Primary antibody
Dilution : 1:200( conc. of dilution )
Incubation Time & Temperature : 4 (℃)/overnight

Reference for 5-Methylcytosine / 5-mC antibody [GT4111]

Application References	Application	Reactivity
Masiello I et al. Ultrastructural localization of 5-methylcytosine on DNA and RNA. Cell Mol Life Sci (Epub ahead of print) (2017).	EM
Hsieh MC et al. Tet1-dependent epigenetic modification of BDNF expression in dorsal horn neurons mediates neuropathic pain in rats. Sci Rep 6:37411 (2016).	Dot
Shih CC et al. Procainamide Inhibits DNA Methylation and Alleviates Multiple Organ Dysfunction in Rats with Endotoxic Shock. PLoS One 11 (9):e0163690 (2016).	IHC-P
Hsiao KY et al. Coordination of AUF1 and miR-148a destabilizes DNA methyltransferase 1 mRNA under hypoxia in endometriosis. Mol Hum Reprod (Epub ahead of print) (2015).	IHC-P

*Resource for General Protocol

*Specific protocol for

Dot blot protocol for GTX629448

*MSDS

MSDS-PBS.pdf

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Secondary Antibodies

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