|Applications||ICC/IF, IHC-Fr, IHC-P, WB|
Not tested in other applications.
|IHC (Frozen sections)||1:100-1:1000*
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||56 kDa (note)
|Positive Controls||SK-N-SH , mouse brain , rat brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||4-aminobutyrate aminotransferase|
|Product Description||Rabbit Polyclonal antibody to ABAT (4-aminobutyrate aminotransferase)|
|Background||4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq]|
|Synonyms||FLJ17813 antibody, FLJ30272 antibody, GABA-AT antibody, GABAT antibody, NPD009 antibody, ABAT antibody, GABA transaminase antibody, 4-aminobutyrate aminotransferase, mitochondrial antibody, (S)-3-amino-2-methylpropionate transaminase antibody, gamma-amino-N-butyrate transaminase antibody, 4-aminobutyrate transaminase antibody, GABA aminotransferase antibody, GABA transferase antibody, 4-aminobutyrate aminotransferase antibody|
|Cellular Localization||Mitochondrion matrix |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human ABAT. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Pig (93%), Bovine (95%)|