ACADM antibody

Cat. No. GTX100032

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all ACADM products

Datasheet
Document

View product citations for antibody GTX100032 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Mouse, Rat, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ACADM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240401

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acyl-CoA dehydrogenase medium chain , ACAD1 , MCAD , MCADH

Cellular Localization

Mitochondrion matrix

Background

This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 34 ACADM
UniProt: P11310 ACADM

Research Area

DATA IMAGES

GTX100032 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
10% SDS PAGE
GTX100032 diluted at 1:1000

GTX100032 ICC/IF Image

ACADM antibody detects ACADM protein at mitochondria by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: ACADM protein stained by ACADM antibody (GTX100032) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX100032 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using ACADM(GTX100032) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Document
Unmodified.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty