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ACADM antibody

Anti-ACADM antibody used in IHC (Paraffin sections) (IHC-P). GTX101393
Anti-ACADM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101393
Anti-ACADM antibody used in IHC (Paraffin sections) (IHC-P). GTX101393
Anti-ACADM antibody used in Western Blot (WB). GTX101393
Anti-ACADM antibody used in Western Blot (WB). GTX101393

Cat. No. GTX101393

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

NIH-3T3 , A431

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACADM. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1240402

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acyl-CoA dehydrogenase medium chain , ACAD1 , MCAD , MCADH

Cellular Localization

Mitochondrion matrix

Background

This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

  • Gene ID: 34 ACADM
  • UniProt: P11310 ACADM

Research Area

DATA IMAGES

Anti-ACADM antibody used in IHC (Paraffin sections) (IHC-P). GTX101393

GTX101393 IHC-P Image

ACADM antibody detects ACADM protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ACADM antibody (GTX101393) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACADM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101393

GTX101393 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using ACADM(GTX101393) antibody at 1:200 dilution.

Anti-ACADM antibody used in IHC (Paraffin sections) (IHC-P). GTX101393

GTX101393 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ACADM(GTX101393) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ACADM antibody used in Western Blot (WB). GTX101393

GTX101393 WB Image

Sample (30 ug of whole cell lysate)
A:NIH-3T3 10% SDS PAGE
GTX101393 diluted at 1:1000

Anti-ACADM antibody used in Western Blot (WB). GTX101393

GTX101393 WB Image

Sample
A: 30μg of A431 whole cell lysate (GTX27909)
7.5% SDS PAGE
GTX101393 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169