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AChR antibody [88B]

Anti-AChR antibody [88B] used in IHC (Paraffin sections) (IHC-P). GTX22804
Anti-AChR antibody [88B] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22804
Anti-AChR antibody [88B] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22804
Anti-AChR antibody [88B] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22804
Anti-AChR antibody [88B] used in IHC (Paraffin sections) (IHC-P). GTX22804
Anti-AChR antibody [88B] used in IHC (Paraffin sections) (IHC-P). GTX22804

Cat. No. GTX22804

Host

Mouse

Clonality

Monoclonal

Clone Name

88B

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA

Reactivity

Human, Mouse, Rat, Rabbit, Chicken, Amphibians, Fish
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5,000
ICC/IF 1:20-100
IHC-P Assay dependent
IHC-Fr 1:1,000
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Product Note

This antibody detects nicotinic acetylcholine receptor (nAChR) gamma and delta subunits. This antibody does not detect the alpha 1 and beta 1 subunits.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

Purified Torpedo californica acetylcholine receptor.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cholinergic receptor nicotinic delta subunit , cholinergic receptor nicotinic gamma subunit , ACHRD , CMS2A , CMS3A , CMS3B , CMS3C , FCCMS , SCCMS , ACHRG

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1’s, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).

Database

Research Area

DATA IMAGES

Anti-AChR antibody [88B] used in IHC (Paraffin sections) (IHC-P). GTX22804

GTX22804 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX22804 AChR antibody [88B].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-AChR antibody [88B] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22804

GTX22804 ICC/IF Image

ICC/IF analysis of formaldehyde fixed U251 cells using GTX22804 AChR antibody [88B]. Cells were probed without (right) or with (left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Dilution : 1:100 overnight at 4 oC

Anti-AChR antibody [88B] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22804

GTX22804 ICC/IF Image

ICC/IF analysis of formaldehyde fixed Neuro-2a cells using GTX22804 AChR antibody [88B]. Cells were probed without (right) or with (left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Dilution : 1:100 overnight at 4 oC

Anti-AChR antibody [88B] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22804

GTX22804 ICC/IF Image

ICC/IF analysis of formaldehyde fixed HeLa cells using GTX22804 AChR antibody [88B]. Cells were probed without (right) or with (left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Dilution : 1:100 overnight at 4 oC

Anti-AChR antibody [88B] used in IHC (Paraffin sections) (IHC-P). GTX22804

GTX22804 IHC-P Image

IHC-P analysis of mouse lymph node tissue using GTX22804 AChR antibody [88B].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-AChR antibody [88B] used in IHC (Paraffin sections) (IHC-P). GTX22804

GTX22804 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX22804 AChR antibody [88B].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

REFERENCE

There are currently no references for AChR antibody [88B] (GTX22804). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for AChR antibody [88B] (GTX22804). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399