AEC Chromogenic kit
|Product Description||AEC Chromogenic kit|
|Background||AEC is one of the most important chromogenic reagents for peroxidase. The coloration which is showed to be fresh red, and applies to immunohistochemistry and various dot detection. Easy to solution in organic impregnant is its defect, to seal slide with water soluble sealing reagent in case of using AEC is recommend.|
|Target||AEC Chromogenic kit|
|Application Note||1. After the peroxidase detection, wash 4 times with TBS or PBS and each time for 5 minutes.|
2. Add one drop of chromogenic reagent A and B respectively into 1ml of distilled water and mix evenly Add it to the sample and colorate in dark for 10-30 minutes. If no background appearing, keep coloration until satisfied.
3. Wash fully with distilled water to stop reaction. Counterstain with methyl blue for 3-5 minutes or nonalcoholic hematoxylin (Mayer's) if necessary.
4. Seal sllide with water soluble sealing reagent (Boster can provide), or glycerol. Observe with microscope. Counterstain with alcoholic hematoxylin is not recommended.
Chromogenic reagent A: AEC concentrated solution, 3ml. Dilution: 1:20
Chromogenic reagent B: acetate concentrated buffer (containing H2O2, pH5.6), 3 ml. Dilution: 1:20.
|Storage Instruction||Store at -20°C for one year.|
|Notes||For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.|