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AHR (phospho Ser36) antibody

Anti-AHR (phospho Ser36) antibody used in Western Blot (WB). GTX52324
Anti-AHR (phospho Ser36) antibody used in Western Blot (WB). GTX52324
Anti-AHR (phospho Ser36) antibody used in ELISA (ELISA). GTX52324
Anti-AHR (phospho Ser36) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52324
Anti-AHR (phospho Ser36) antibody used in IHC (Paraffin sections) (IHC-P). GTX52324

Cat. No. GTX52324

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all AHR products

APPLICATION

Application Note

WB 1:500-1:2000, IHC 1:50-1:200

Calculated MW

96 kDa. ( Note )

Product Note

AHR

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human AhR around the phosphorylation site of Ser36

Purification

Antigen affinity purification

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aryl hydrocarbon receptor , bHLHe76

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-AHR (phospho Ser36) antibody used in Western Blot (WB). GTX52324

GTX52324 WB Image

WB analysis of various samples in absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide) using GTX52324 AHR (phospho Ser36) antibody.

Anti-AHR (phospho Ser36) antibody used in Western Blot (WB). GTX52324

GTX52324 WB Image

WB analysis of HepG2 cell lysates using GTX52324 AHR (phospho Ser36) antibody.
Left : Antibody prior incubated with antigen-specific peptide
Right : Primary antibody

Anti-AHR (phospho Ser36) antibody used in ELISA (ELISA). GTX52324

GTX52324 ELISA Image

ELISA analysis of phospho-peptide (P-peptide) and non-phospho-peptide(N-peptide) using GTX52324 AHR (phospho Ser36) antibody.
Peptides concentration : 1ug/ml

Anti-AHR (phospho Ser36) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52324

GTX52324 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells using GTX52324 AHR (phospho Ser36) antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200 incubated with the sample for 1 hour at 37ºC

Anti-AHR (phospho Ser36) antibody used in IHC (Paraffin sections) (IHC-P). GTX52324

GTX52324 IHC-P Image

IHC-P analysis of human brain tissue sections using GTX52324 AHR (phospho Ser36) antibody.
Dilution : 1:200
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399