APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
IMR32 , 293T
Predict Reactivity
Mouse, Rat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 40% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human AKAP1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240436
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
A-kinase anchoring protein 1 , AKAP , AKAP121 , AKAP149 , AKAP84 , D-AKAP1 , PPP1R43 , PRKA1 , SAKAP84 , TDRD17
Cellular Localization
Mitochondrion outer membrane
Background
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-AKAP1 antibody [C2C3], C-term used in Western Blot (WB). GTX102578](/templates/default/js/lightbox/images/loading.gif "Anti-AKAP1 antibody [C2C3], C-term used in Western Blot (WB). GTX102578")
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GTX102578 WB Image
Wild-type (WT) and AKAP1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKAP1 antibody [C2C3], C-term (GTX102578) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX102578 WB Image
Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX102578 diluted at 1:1000
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REFERENCE
There are currently no references for AKAP1 antibody [C2C3], C-term (GTX102578). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for AKAP1 antibody [C2C3], C-term (GTX102578). Be the first to share your experience with this product.
![Anti-AKAP1 antibody [C2C3], C-term used in Western Blot (WB). GTX102578](/upload/website/prouct_img/small/GTX102578/GTX102578_39680_20180209_WB_KO_watermark_w_23060100_287.webp "Anti-AKAP1 antibody [C2C3], C-term used in Western Blot (WB). GTX102578")
![Anti-AKAP1 antibody [C2C3], C-term used in Western Blot (WB). GTX102578](/upload/website/prouct_img/small/GTX102578/GTX102578_39680_WB_w_23060100_954.webp "Anti-AKAP1 antibody [C2C3], C-term used in Western Blot (WB). GTX102578")
![Anti-AKAP1 antibody [C2C3], C-term used in Western Blot (WB). GTX102578](/upload/website/prouct_img/normal/GTX102578/GTX102578_39680_20180209_WB_KO_watermark_w_23060100_287.webp "Anti-AKAP1 antibody [C2C3], C-term used in Western Blot (WB). GTX102578")
![Anti-AKAP1 antibody [C2C3], C-term used in Western Blot (WB). GTX102578](/upload/website/prouct_img/normal/GTX102578/GTX102578_39680_WB_w_23060100_954.webp "Anti-AKAP1 antibody [C2C3], C-term used in Western Blot (WB). GTX102578")