AKAP12 antibody [C3], C-term
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||191 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLaS3 , HepG2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||A kinase (PRKA) anchor protein 12|
|Product Description||Rabbit polyclonal antibody to AKAP12 (A kinase (PRKA) anchor protein (gravin) 12)|
|Background||The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]|
|Synonyms||AKAP250 antibody, DKFZp686M0430 antibody, DKFZp686O0331 antibody, FLJ20945 antibody, FLJ97621 antibody, AKAP12 antibody, Src-Suppressed C Kinase Substrate antibody, "A-kinase anchor protein, 250kDa antibody", myasthenia gravis autoantigen gravin antibody, A-kinase anchor protein 12 antibody, kinase scaffold protein gravin antibody, AKAP 250 antibody, A kinase (PRKA) anchor protein 12 antibody|
|Cellular Localization||Cytoplasm , cell cortex , cytoskeleton |
|Immunogen||Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AKAP12. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Rhesus Monkey (100%)|