APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1,000 |
1:500 |
1-10 μl |
Not tested in other applications.
Product Note
This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)).
PROPERTIES
Form
Liquid
Buffer
Serum
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
The AML1-ETO fusion protein using a KLH-conjugated synthetic peptide.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
runt related transcription factor 1 , RUNX1 translocation partner 1 , AML1 , AML1-EVI-1 , AMLCR1 , CBF2alpha , CBFA2 , EVI-1 , PEBP2aB , PEBP2alpha , AML1-MTG8 , AML1T1 , CBFA2T1 , CDR , ETO , MTG8 , ZMYND2
Cellular Localization
Nucleus
Database
Research Area
DATA IMAGES
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GTX60339 WB Image
WB analysis of nuclear extracts of SKNO-1 cells (15 μg) using GTX60339 AML1-ETO antibody - ChIP grade. Dilution : 1:1,000
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GTX60339 ELISA Image
ELISA analysis of peptides used for immunization using GTX60339 AML1-ETO antibody - ChIP grade.
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GTX60339 ChIP assay Image
ChIP analysis of sheared chromatin from 1.25x10⁶ Kasumi-1 cells using GTX60339 AML1-ETO antibody - ChIP grade. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. This figure shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used. Antibody amount : 4μl
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REFERENCE
There are currently no references for AML1-ETO antibody - ChIP grade (GTX60339). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for AML1-ETO antibody - ChIP grade (GTX60339). Be the first to share your experience with this product.