AMPK alpha 1 (phospho Ser496) antibody

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GTX32345

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100 μl	USD 319.00

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Catalog Number	GTX32345 ☆☆☆☆☆ ( 0 ) ( 0 )
Product Name	AMPK alpha 1 (phospho Ser496) antibody See all AMPK alpha 1 products
Applications	ICC/IF, IHC-P, WB
Application Note	WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. ICC/IF: 1:100 - 1:500
Predicted Target Size	64 kDa (note)
Form Supplied	Liquid
Purification	The antibody was purified by immunogen affinity chromatography.

Full Name	protein kinase, AMP-activated, alpha 1 catalytic subunit
Background	The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Synonyms	AMPKa1 Antibody , PRKAA1 Antibody , AMPK Antibody , protein kinase, AMP-activated, alpha 1 catalytic subunit Antibody
Specificity	Recognizes endogenous levels of AMPK alpha 1 (pS496) protein.
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human AMPK alpha 1 (pS496). The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human, Mouse, Rat
Conjugation	Unconjugated

Storage Buffer	Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.
Storage Instruction	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Cancer > Hypoxia Cell Biology > Apoptosis > Anti-apoptosis Disease Related > Alzheimer's disease Disease Related > Cardiovascular > Heart > Heart disease More Hide

AMPK alpha 1 (phospho Ser496) antibody validated data

GTX32345 WB Image

Western blot analysis of AMPK alpha 1 (pS496) expression in HUVEC UV-treated (A); HeLa (B); SP2/0 (C); PC12 (D) whole cell lysates.

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GTX32345 IHC-P Image

Immunohistochemical analysis of AMPK alpha 1 (pS496) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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GTX32345 ICC/IF Image

Immunofluorescent analysis of AMPK alpha 1 (pS496) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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GTX32345 WB Image

Western blot analysis of AMPK alpha 1 (pS496) expression in HUVEC UV-treated (A); HeLa (B); SP2/0 (C); PC12 (D) whole cell lysates.

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GTX32345 IHC-P Image

Immunohistochemical analysis of AMPK alpha 1 (pS496) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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GTX32345 ICC/IF Image

Immunofluorescent analysis of AMPK alpha 1 (pS496) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Primary Antibodies