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  • AMPK alpha 1 (phospho Ser496) antibody

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1 .GTX32345 AMPK alpha 1 (phospho Ser496) antibody

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AMPK alpha 1 (phospho Ser496) antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman, Mouse, Rat
Cat No: GTX32345
Package Price Qty
100 μlUSD 319.00
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  • Anti-AMPK alpha 1 (phospho Ser496) antibody used in Western Blot (WB). GTX32345
  • Anti-AMPK alpha 1 (phospho Ser496) antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX32345
  • Anti-AMPK alpha 1 (phospho Ser496) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32345
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  • Datasheet
  • Application Information: AMPK alpha 1 (phospho Ser496) antibody

Catalog Number GTX32345
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Product Name AMPK alpha 1 (phospho Ser496) antibody
See all AMPK alpha 1 products
ApplicationsICC/IF, IHC-P, WB
Application NoteWB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. ICC/IF: 1:100 - 1:500
Form SuppliedLiquid
PurificationThe antibody was purified by immunogen affinity chromatography.

  • Specifications: AMPK alpha 1 (phospho Ser496) antibody

Full Nameprotein kinase, AMP-activated, alpha 1 catalytic subunit
BackgroundThe protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
SynonymsAMPKa1 Antibody , PRKAA1 Antibody , AMPK Antibody , protein kinase, AMP-activated, alpha 1 catalytic subunit Antibody
SpecificityRecognizes endogenous levels of AMPK alpha 1 (pS496) protein.
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human AMPK alpha 1 (pS496). The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Rat
ConjugationUnconjugated

  • Storage Conditions: AMPK alpha 1 (phospho Ser496) antibody

Storage BufferLiquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.
Storage InstructionShipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Hypoxia
  • Cell Biology > Apoptosis > Anti-apoptosis
  • Disease Related > Alzheimer's disease
  • Disease Related > Cardiovascular > Heart > Heart disease
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AMPK alpha 1 (phospho Ser496) antibody validated data
  • Anti-AMPK alpha 1 (phospho Ser496) antibody used in Western Blot (WB). GTX32345
    • GTX32345 WB Image
      Western blot analysis of AMPK alpha 1 (pS496) expression in HUVEC UV-treated (A); HeLa (B); SP2/0 (C); PC12 (D) whole cell lysates.
      Top
  • Anti-AMPK alpha 1 (phospho Ser496) antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX32345
    • GTX32345 IHC-P Image
      Immunohistochemical analysis of AMPK alpha 1 (pS496) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
      Top
  • Anti-AMPK alpha 1 (phospho Ser496) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32345
    • GTX32345 ICC/IF Image
      Immunofluorescent analysis of AMPK alpha 1 (pS496) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
      Top
  • Anti-AMPK alpha 1 (phospho Ser496) antibody used in Western Blot (WB). GTX32345
    • GTX32345 WB Image
      Western blot analysis of AMPK alpha 1 (pS496) expression in HUVEC UV-treated (A); HeLa (B); SP2/0 (C); PC12 (D) whole cell lysates.
      Top
  • Anti-AMPK alpha 1 (phospho Ser496) antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX32345
    • GTX32345 IHC-P Image
      Immunohistochemical analysis of AMPK alpha 1 (pS496) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
      Top
  • Anti-AMPK alpha 1 (phospho Ser496) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32345
    • GTX32345 ICC/IF Image
      Immunofluorescent analysis of AMPK alpha 1 (pS496) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
      Top
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