AP2 gamma antibody, C-term
|Application Note||WB experiments gave bands at approx 75kDa and 22kDa in Human Placenta and Ovary lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 49.2kDa according to NP_003213.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? In paraffin embedded Human Skin shows nuclear staining of keratinocytes. Recommended concentration, 2-3µg/ml. |
|Positive Controls||The peptide used to product this antibody is available for purchase (Catalog Number:GTX89567-PEP).|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Full Name||transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)|
|Product Description||Goat polyclonal antibody to AP-2 gamma|
|Background||Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. |
|Synonyms||transcription factor AP2 gamma (activating en, transcription factor AP-2 gamma (activating enhancer-binding protein 2 gamma), AP2 gamma, transcription factor AP2 gamma (activating enhancerbinding protein2 gamma), hAP2g, AP2GAMMA, AP2-GAMMA, TFAP2G, hAP-2g, transcription factor AP-2 gamma (activating en, TFAP2C, transcription factor AP2 gamma (activating enhancerbinding protein 2 gamma), ERF1, hAP 2g|
|Specificity||This antibody is specific for AP-2 gamma.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Mouse, Dog, Pig, Bovine|