|Applications||ICC/IF, IHC-P, IP, WB, ChIP assay|
Not tested in other applications.
| ChIP assay||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||36 kDa (note)
|Positive Controls||A431 , HeLa , Molt-4 , mouse brain , PC-12|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||APEX nuclease (multifunctional DNA repair enzyme) 1|
|Product Description||Rabbit Polyclonal antibody to APE1 (APEX nuclease (multifunctional DNA repair enzyme) 1)|
|Background||Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq]|
|Synonyms||APE antibody, APE1 antibody, APEN antibody, APEX antibody, APX antibody, HAP1 antibody, REF1 antibody, APEX1 antibody, apurinic-apyrimidinic endonuclease 1 antibody, deoxyribonuclease (apurinic or apyrimidinic) antibody, apurinic/apyrimidinic (abasic) endonuclease antibody, redox factor-1 antibody, protein REF-1 antibody, AP endonuclease class I antibody, AP lyase antibody, DNA-(apurinic or apyrimidinic site) lyase antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 antibody|
|Cellular Localization||Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human APE1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Dog, Pig, Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Dog (95%), Pig (94%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (93%)|