|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||21 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , H1299 , Mouse brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||ADP-ribosylation factor 1|
|Product Description||Rabbit Polyclonal antibody to ARF1 (ADP-ribosylation factor 1)|
|Background||ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]|
|Synonyms||ARF1 antibody, ADP-ribosylation factor 1 antibody|
|Cellular Localization||Golgi apparatus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human ARF1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Zebrafish, Xenopus laevis, Dog, Pig, Chicken, Sheep, Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Rat (100%), Zebrafish (98%), Xenopus laevis (99%), Dog (94%), Pig (100%), Chicken (98%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%)|