|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Positive Controls||293T , A549 , HepG2 , HepG2 nuclear , A431 , HeLa|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||AT rich interactive domain 1A (SWI-like)|
|Product Description||Rabbit Polyclonal antibody to ARID1A (AT rich interactive domain 1A (SWI-like))|
|Background||This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]|
|Synonyms||BAF250 Antibody, C1orf4 Antibody, AT rich interactive domain 1A (SWI-like) Antibody, B120 Antibody, P270 Antibody, SMARCF1 Antibody, ELD Antibody, OSA1 Antibody, hELD Antibody, hOSA1 Antibody, BAF250a Antibody, MRD14 Antibody, BM029 Antibody|
|Cellular Localization||Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human ARID1A. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Bovine|
|Predict Reactivity Note||Mouse (81%), Rat (81%), Bovine (84%)|