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ATG9A antibody

Anti-ATG9A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128427
Anti-ATG9A antibody used in Immunoprecipitation (IP). GTX128427
Anti-ATG9A antibody used in Western Blot (WB). GTX128427
Anti-ATG9A antibody used in IHC (Paraffin sections) (IHC-P). GTX128427
Anti-ATG9A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128427
Anti-ATG9A antibody used in Western Blot (WB). GTX128427
Anti-ATG9A antibody used in Western Blot (WB). GTX128427
Anti-ATG9A antibody used in Western Blot (WB). GTX128427
Anti-ATG9A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128427
Anti-ATG9A antibody used in Western Blot (WB). GTX128427

Cat. No. GTX128427

Reference 7
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ATG9A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

PC-12 , Rat-2 , BCL-1 , RD

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ATG9A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885776

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 9A , APG9L1 , MGD3208 , mATG9

Cellular Localization

Cytoplasmic vesicle , autophagosome membrane

Background

Plays a role in autophagy.

Database

Research Area

DATA IMAGES

Anti-ATG9A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128427

GTX128427 ICC/IF Image

ATG9A antibody detects ATG9A protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ATG9A stained by ATG9A antibody (GTX128427) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ATG9A antibody used in Immunoprecipitation (IP). GTX128427

GTX128427 IP Image

ATG9A antibody immunoprecipitates ATG9A protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of ATG9A protein by 4 μg ATG9A antibody (GTX128427)
7.5 % SDS-PAGE
The immunoprecipitated ATG9A protein was detected by ATG9A antibody (GTX128427) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-ATG9A antibody used in Western Blot (WB). GTX128427

GTX128427 WB Image

ATG9A antibody detects ATG9A protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG9A antibody (GTX128427) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATG9A antibody used in IHC (Paraffin sections) (IHC-P). GTX128427

GTX128427 IHC-P Image

ATG9A antibody detects ATG9A protein on colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
ATG9A antibody (GTX128427) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ATG9A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128427

GTX128427 ICC/IF Image

ATG9A antibody detects ATG9A protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 100% MeOH for 5 min.
Green: ATG9A protein stained by ATG9A antibody (GTX128427) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ATG9A antibody used in Western Blot (WB). GTX128427

GTX128427 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG9A antibody (GTX128427) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATG9A antibody used in Western Blot (WB). GTX128427

GTX128427 WB Image

ATG9A antibody detects ATG9A protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
7.5% SDS-PAGE
ATG9A antibody (GTX128427) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATG9A antibody used in Western Blot (WB). GTX128427

GTX128427 WB Image

ATG9A antibody detects ATG9A protein by western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
7.5% SDS-PAGE
ATG9A antibody (GTX128427) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATG9A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128427

GTX128427 ICC/IF Image

The data was published in the journal Nat Commun in 2016. PMID: 27510922

Anti-ATG9A antibody used in Western Blot (WB). GTX128427

GTX128427 WB Image

The data was published in the journal PLoS One in 2017. PMID: 28910396

REFERENCE

Application Reference

Buono R et al. Cancers (Basel) 2023; 15 (24) Fasting-Mimicking Diet Inhibits Autophagy and Synergizes with Chemotherapy to Promote T-Cell-Dependent Leukemia-Free Survival.
Zhang X et al. Autophagy 2019; 15 (10) : 1787-1800 GORASP2/GRASP55 collaborates with the PtdIns3K UVRAG complex to facilitate autophagosome-lysosome fusion.
Alejandro Martorell Riera et al. bioRxiv 2019; (Epub) Dynamin-2 controls phagophore maturation.
Prabakaran T et al. EMBO J 2018; (Epub) Attenuation of cGAS-STING signaling is mediated by a p62/SQSTM1-dependent autophagy pathway activated by TBK1.
Application : ICC/IF
Reactivity : Human
Merrill NM et al. PLoS One 2017; 12 (9) : e0184909 PI3K-C2α knockdown decreases autophagy and maturation of endocytic vesicles.
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Submit a Reference

REVIEW

ATG9A antibody Cat. No. GTX128427

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-ATG9A antibody used in IHC (Paraffin sections) (IHC-P). GTX128427
Date : Anonymous submitted on 14-Dec-2022
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : mouse dorsal root ganglia
Blocking : 0.5% skim milk in PBST
Fixation : 4% PFA
Primary Antibody : 1:1000, 4°C, 18Hr
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169