ATM antibody [2C1] (supernatant)
|Applications||ICC/IF, IHC, IHC-P, IP, WB|
|Application Note||Recommended Starting Dilutions:|
For WB: Use at 1:100.
For IP: Use at an assay dependent dilution.
For IHC: Use at 1:50. Analysis was performed of the cerebral cortex of E14.5 wild-type (+/+) and scid mice - (MC Vemuri, 2001) For immunofluorescence please refer to the publication by Harry Scherthan, et.al., 2000 and Yiyoung Liu, et.al., 2006.
For ICC/IF: Use at 1:500.
Optimal dilutions/concentrations should be determined by the researcher.
|Positive Controls||Raji and lymphoblastoid nuclear lysate|
|Concentration||tissue culture supernatant
|Purification Note||From hybridoma culture supernatant|
|Full Name||ataxia telangiectasia mutated|
|Product Description||Mouse monoclonal [2C1] to ATM (supernatant)|
|Background||ATM-2C1 recognizes full-length ATM, a 370kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation.|
|Synonyms||114480, ATC, DKFZp781A0353, Q13315, MGC74674, ATD, ATA, AT1, ATE, 472, ATM, TEL1, ATDC|
|Immunogen||Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.|
|Species Reactivity||Human, Mouse, Monkey, Rat|