ATP5B antibody [4.3E8.D10]

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GTX25432

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100 μg	USD 319.00

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Catalog Number	GTX25432 ☆☆☆☆☆ ( 0 ) ( 0 )
Product Name	ATP5B antibody [4.3E8.D10] See all ATP5B products
Applications	ICC/IF, IP, WB
Application Note	Recommended Starting Dilutions: ICC/IF: 1:100 - 1:1000 WB: 1-2 µg/ml IP: 2-5 ug Optimal dilution / concentration should be determined by the end user.
Form Supplied	Liquid
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration)
Purification	purified

Full Name	ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Background	beta subunit of rat liver ATP synthase [RGD, Feb 2006]
Synonyms	ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide Antibody , Atp5b Antibody
Host	Mouse
Clonality	Monoclonal
Clone Name	4.3E8.D10
Isotype	IgG1
Immunogen	Intact rat mitochondria.
Antigen Species	Rat
Species Reactivity	Human, Mouse, Rat
Conjugation	Unconjugated

Storage Buffer	PBS containing 0.05 % sodium azide and 1 mg/ml BSA
Storage Instruction	-20° C, Avoid Freeze/Thaw Cycles
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Cancer > Angiogenesis Cell Biology > Cell adhesion > Regulation > Negative regulation Disease Related > Cardiovascular > Angiogenesis Metabolism > Lipid and lipoprotein metabolism More Hide

ATP5B antibody [4.3E8.D10] validated data

GTX25432 WB Image

Immunoprecipitation of ATP Synthase beta from rat neuronal/glial cell extract.

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GTX25432 ICC/IF Image

Indirect immunofluorescence staining of rat neuronal/glial cell culture.

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GTX25432 IP Image

Immunoprecipitation of ATP Synthase beta in THP-1 cells. Antigen-antibody complexes were formed by incubating 500ug of THP-1 whole cell lysate (in 500ul volume) with 5ul of ATP Synthase beta antibody [4.3E8.D10] overnight at 4°C. The immune complexes were captured on 30ul of protein G agarose, washed extensively, and eluted. Samples were resolved on an 8% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with ATP Synthase beta antibody [4.3E8.D10] at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.

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GTX25432 WB Image

Immunoprecipitation of ATP Synthase beta from rat neuronal/glial cell extract.

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GTX25432 ICC/IF Image

Indirect immunofluorescence staining of rat neuronal/glial cell culture.

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GTX25432 IP Image

Immunoprecipitation of ATP Synthase beta in THP-1 cells. Antigen-antibody complexes were formed by incubating 500ug of THP-1 whole cell lysate (in 500ul volume) with 5ul of ATP Synthase beta antibody [4.3E8.D10] overnight at 4°C. The immune complexes were captured on 30ul of protein G agarose, washed extensively, and eluted. Samples were resolved on an 8% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with ATP Synthase beta antibody [4.3E8.D10] at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.

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Primary Antibodies