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  • ATP5B antibody [4.3E8.D10]

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1 .GTX25432 ATP5B antibody [4.3E8.D10]

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ATP5B antibody [4.3E8.D10]


  • Host / ClonalityMouse Monoclonal
  • Clone Name4.3E8.D10
  • ApplicationsICC/IF, IP, WB
  • Species reactivityHuman, Mouse, Rat
Cat No: GTX25432
Package Price Qty
100 μgUSD 319.00
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  • Anti-ATP5B antibody [4.3E8.D10] used in Western Blot (WB). GTX25432
  • Anti-ATP5B antibody [4.3E8.D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25432
  • Anti-ATP5B antibody [4.3E8.D10] used in Immunoprecipitation. GTX25432
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  • Datasheet
  • Application Information: ATP5B antibody [4.3E8.D10]

Catalog Number GTX25432
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Product Name ATP5B antibody [4.3E8.D10]
See all ATP5B products
ApplicationsICC/IF, IP, WB
Application NoteRecommended Starting Dilutions:
ICC/IF: 1:100 - 1:1000
WB: 1-2 µg/ml
IP: 2-5 ug Optimal dilution / concentration should be determined by the end user.
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
Purificationpurified

  • Specifications: ATP5B antibody [4.3E8.D10]

Full NameATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Backgroundbeta subunit of rat liver ATP synthase [RGD, Feb 2006]
SynonymsATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide Antibody , Atp5b Antibody
HostMouse
ClonalityMonoclonal
Clone Name4.3E8.D10
IsotypeIgG1
ImmunogenIntact rat mitochondria.
Antigen SpeciesRat
Species ReactivityHuman, Mouse, Rat
ConjugationUnconjugated

  • Storage Conditions: ATP5B antibody [4.3E8.D10]

Storage BufferPBS containing 0.05 % sodium azide and 1 mg/ml BSA
Storage Instruction-20° C, Avoid Freeze/Thaw Cycles
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Angiogenesis
  • Cell Biology > Cell adhesion > Regulation > Negative regulation
  • Disease Related > Cardiovascular > Angiogenesis
  • Metabolism > Lipid and lipoprotein metabolism
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ATP5B antibody [4.3E8.D10] validated data
  • Anti-ATP5B antibody [4.3E8.D10] used in Western Blot (WB). GTX25432
    • GTX25432 WB Image
      Immunoprecipitation of ATP Synthase beta from rat neuronal/glial cell extract.
      Top
  • Anti-ATP5B antibody [4.3E8.D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25432
    • GTX25432 ICC/IF Image
      Indirect immunofluorescence staining of rat neuronal/glial cell culture.
      Top
  • Anti-ATP5B antibody [4.3E8.D10] used in Immunoprecipitation. GTX25432
    • GTX25432 IP Image
      Immunoprecipitation of ATP Synthase beta in THP-1 cells. Antigen-antibody complexes were formed by incubating 500ug of THP-1 whole cell lysate (in 500ul volume) with 5ul of ATP Synthase beta antibody [4.3E8.D10] overnight at 4°C. The immune complexes were captured on 30ul of protein G agarose, washed extensively, and eluted. Samples were resolved on an 8% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with ATP Synthase beta antibody [4.3E8.D10] at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.
      Top
  • Anti-ATP5B antibody [4.3E8.D10] used in Western Blot (WB). GTX25432
    • GTX25432 WB Image
      Immunoprecipitation of ATP Synthase beta from rat neuronal/glial cell extract.
      Top
  • Anti-ATP5B antibody [4.3E8.D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25432
    • GTX25432 ICC/IF Image
      Indirect immunofluorescence staining of rat neuronal/glial cell culture.
      Top
  • Anti-ATP5B antibody [4.3E8.D10] used in Immunoprecipitation. GTX25432
    • GTX25432 IP Image
      Immunoprecipitation of ATP Synthase beta in THP-1 cells. Antigen-antibody complexes were formed by incubating 500ug of THP-1 whole cell lysate (in 500ul volume) with 5ul of ATP Synthase beta antibody [4.3E8.D10] overnight at 4°C. The immune complexes were captured on 30ul of protein G agarose, washed extensively, and eluted. Samples were resolved on an 8% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with ATP Synthase beta antibody [4.3E8.D10] at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.
      Top
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