|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||68 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , mouse brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A|
|Product Description||Rabbit Polyclonal antibody to ATP6V1A (ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A)|
|Background||This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq]|
|Synonyms||ATP6A1 antibody, ATP6V1A1 antibody, HO68 antibody, VA68 antibody, VPP2 antibody, Vma1 antibody, ATP6V1A antibody, ATPase, H+ transporting, lysosomal, subunit A1 antibody, V-type proton ATPase catalytic subunit A antibody, vacuolar ATP synthase catalytic subunit A, ubiquitous isoform antibody, H(+)-transporting two-sector ATPase, subunit A antibody, vacuolar ATPase isoform VA68 antibody, V-ATPase 69 kDa subunit 1 antibody, V-ATPase A subunit 1 antibody, V-ATPase subunit A antibody, H+-transporting ATPase chain A, vacuolar (VA68 type) antibody, vacuolar proton pump subunit alpha antibody, vacuolar proton pump alpha subunit 1 antibody, ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human ATP6V1A. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Rat (95%), Xenopus laevis (90%), Pig (96%), Chicken (83%), Rhesus Monkey (99%), Bovine (98%)|