Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Positive Controls||U87-MG , SK-N-SH , IMR32 , SK-N-AS , Mouse brain , Rat brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2|
|Product Description||Rabbit Polyclonal antibody to ATP6V1B2 (ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2)|
|Background||This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq]|
|Synonyms||ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 Antibody, VATB Antibody, VPP3 Antibody, ATP6B2 Antibody, HO57 Antibody, ATP6B1B2 Antibody, Vma2 Antibody|
|Cellular Localization||Endomembrane system; Peripheral membrane protein , Melanosome |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human ATP6V1B2. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Chicken, Chimpanzee, Bovine|
|Predict Reactivity Note||Zebrafish (96%), Chicken (99%), Chimpanzee (99%), Bovine (100%)|