ATP6V1H antibody [N3C3]
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
| Immunocytochemistry/ Immunofluorescence||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||56 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , Raji , mouse brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H|
|Product Description||Rabbit Polyclonal antibody to ATP6V1H (ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H)|
|Background||This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the regulatory H subunit of the V1 domain which is required for catalysis of ATP but not the assembly of V-ATPase. Three alternatively spliced transcript variants encode two isoforms of the H subunit. [provided by RefSeq]|
|Synonyms||MSTP042 antibody, NBP1 antibody, SFD antibody, SFDalpha antibody, SFDbeta antibody, VMA13 antibody, ATP6V1H antibody, protein VMA13 homolog antibody, V-type proton ATPase subunit H antibody, V-ATPase subunit H antibody, nef-binding protein 1 antibody, vacuolar proton pump subunit H antibody, vacuolar proton pump H subunit antibody, vacuolar ATP synthase subunit H antibody, V-ATPase H subunit antibody, vacuolar proton pump subunit SFD antibody, vacuolar ATPase subunit H antibody, V-ATPase 50/57 kDa subunits antibody, "ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H antibody"|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human ATP6V1H. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Xenopus laevis, Pig, Chicken, Bovine|
|Predict Reactivity Note||Zebrafish (91%), Xenopus laevis (94%), Pig (99%), Chicken (96%), Bovine (98%)|