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Acetyl Lysine antibody [1C6]

Anti-Acetyl Lysine antibody [1C6] used in ChIP assay (ChIP assay). GTX80693
Anti-Acetyl Lysine antibody [1C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80693
Anti-Acetyl Lysine antibody [1C6] used in Western Blot (WB). GTX80693

Cat. No. GTX80693

Host

Mouse

Clonality

Monoclonal

Clone Name

1C6

Isotype

IgG

Application

WB, ICC/IF, FACS, IP, Dot, ChIP assay

Reactivity

Species independent
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:100 - 1:500
FACS Assay dependent
IP 3 μg
Dot Assay dependent
ChIP assay 10 μg
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide sequence surrounding the acetylated lysine 9 of histone H3.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_625365

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.

Research Area

DATA IMAGES

Anti-Acetyl Lysine antibody [1C6] used in ChIP assay (ChIP assay). GTX80693

GTX80693 ChIP assay Image

ChIP analysis of 4x10⁶ LNCaP cells using GTX80693 Acetyl Lysine antibody [1C6]. Quantitation of immunoprecipitated GAPDH promoter sequence is presented as fold enrichment of the acetyl lysine monoclonal antibody versus non-specific IgG.

Anti-Acetyl Lysine antibody [1C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80693

GTX80693 ICC/IF Image

ICC/IF analysis of HeLa cells untreated (left panels) or treated with 0.3uM Trichostatin A for 16 hours (right panels) using GTX80693 Acetyl Lysine antibody [1C6].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes

Anti-Acetyl Lysine antibody [1C6] used in Western Blot (WB). GTX80693

GTX80693 WB Image

WB analysis of untreated (DMSO only) or cells treated with 0.3uM or 3uM of Trichostatin A (TSA) for 16 hours using GTX80693 Acetyl Lysine antibody [1C6].
Dilution : 1:1000

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399