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  • Adenovirus type 5 E1A antibody [0.N.8]

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1 .GTX51686 Adenovirus type 5 E1A antibody [0.N.8]

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Adenovirus type 5 E1A antibody [0.N.8]


  • Host / ClonalityMouse Monoclonal
  • Clone Name0.N.8
  • ApplicationsICC/IF, IHC-F, IP, WB
  • Species reactivityAdenovirus
Cat No: GTX51686
Package Price Qty
250 μlUSD 319.00
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  • Datasheet
  • Application Information: Adenovirus type 5 E1A antibody [0.N.8]

Catalog Number GTX51686
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Product Name Adenovirus type 5 E1A antibody [0.N.8]
See all Adenovirus E1A products
ApplicationsICC/IF, IHC-F, IP, WB
Application NoteIF: Use at a concentration of 0.2 - 0.4 μg/ml. IHC-F: Use at a concentration of 2 - 4 μg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6, 10-20 min, followed by cooling at RT 20 min.IP: Use Protein A; 2ug antibody/mg protein lysateWB: Use at a concentration of 0.5 - 1.0 μg/ml for 2 hrs at RT, Predicted molecular weight: 35-46 kDa. Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Positive Controls293 cells or adenovirus infected cells and tissues.
Form SuppliedLiquid
Concentration0.2 mg/ml (Please refer to the vial label for the specific concentration)
PurificationProtein A purified

  • Specifications: Adenovirus type 5 E1A antibody [0.N.8]

BackgroundThe early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
Cellular LocalizationNuclear
Specificityrecognises Adenovirus, Type 5
HostMouse
ClonalityMonoclonal
Clone Name0.N.8
IsotypeIgG2a
ImmunogenTissue / cell preparation Adenovirus.
Light Chainkappa
Species ReactivityAdenovirus
ConjugationUnconjugated

  • Storage Conditions: Adenovirus type 5 E1A antibody [0.N.8]

Storage BufferPreservative: 15mM Sodium Azide Constituents: 0.2% BSA, 10mM PBS. pH 7.4
Storage InstructionStore at +4°C short term. Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Infectious Disease > Virus > Adenovirus
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Adenovirus type 5 E1A antibody [0.N.8] validated data
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