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  • Adenylate kinase 2 antibody [N1C3-2]

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1 .GTX111737 Adenylate kinase 2 antibody [N1C3-2]

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Adenylate kinase 2 antibody [N1C3-2]

Knockout/Knockdown

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman, Mouse, Rat
Cat No: GTX111737
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in Western Blot (WB). GTX111737
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX111737
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX111737
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX111737
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in Western Blot (WB). GTX111737
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111737
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in Western Blot (WB). GTX111737
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  • Application Information: Adenylate kinase 2 antibody [N1C3-2]

Catalog Number GTX111737
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Product Name Adenylate kinase 2 antibody [N1C3-2]
See all Adenylate kinase 2 products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size26 kDa (note) IsoForm (7)
Positive Controls293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Adenylate kinase 2 antibody [N1C3-2]

Full Nameadenylate kinase 2
BackgroundAdenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
SynonymsADK2 antibody, AK 2 antibody, AK2 antibody, ATP-AMP transphosphorylase 2 antibody, "adenylate kinase 2, mitochondrial antibody", "adenylate kinase isoenzyme 2, mitochondrial antibody", adenylate kinase 2 antibody
Cellular LocalizationMitochondrion intermembrane space
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human Adenylate kinase 2. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rhesus Monkey, Bovine
Predict Reactivity NoteRhesus Monkey (100%), Bovine (95%)
ConjugationUnconjugated

  • Storage Conditions: Adenylate kinase 2 antibody [N1C3-2]

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Adenylate kinase 2 antibody [N1C3-2] validated data
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in Western Blot (WB). GTX111737
    • GTX111737 WB Image  Knockout/Knockdown
      Wild-type (WT) and Adenylate kinase 2 knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Adenylate kinase 2 antibody (GTX111737) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      CRISPR knockout cell lines generated by www.edigene.com
      Top
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX111737
    • GTX111737 IHC-P Image
      Adenylate kinase 2 antibody [N1C3-2] detects Adenylate kinase 2 protein at mitochondria in rat ovary by immunohistochemical analysis.
      Sample: Paraffin-embedded rat ovary.
      Adenylate kinase 2 antibody [N1C3-2] (GTX111737) diluted at 1:500.
      Top
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX111737
    • GTX111737 IHC-P Image
      Adenylate kinase 2 antibody [N1C3-2] detects Adenylate kinase 2 protein at mitochondria in rat pancreas by immunohistochemical analysis.
      Sample: Paraffin-embedded rat pancreas.
      Adenylate kinase 2 antibody [N1C3-2] (GTX111737) diluted at 1:500.
      Top
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX111737
    • GTX111737 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Adenylate kinase 2(GTX111737) antibody at 1:500 dilution.
      Top
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in Western Blot (WB). GTX111737
    • GTX111737 WB Image
      Sample (50 ug of whole cell lysate)
      A: mouse brain
      12% SDS PAGE
      GTX111737 diluted at 1:1000
      Top
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111737
    • GTX111737 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AK2(GTX111737) antibody at 1:200 dilution.
      Top
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in Western Blot (WB). GTX111737
    • GTX111737 WB Image
      Sample (30 ug of whole cell lysate)
      A: Molt-4 (GTX27912)
      12% SDS PAGE
      GTX111737 diluted at 1:1000
      Top
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in Western Blot (WB). GTX111737
    • GTX111737 WB Image  Knockout/Knockdown
      Wild-type (WT) and Adenylate kinase 2 knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Adenylate kinase 2 antibody (GTX111737) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      CRISPR knockout cell lines generated by www.edigene.com
      Top
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX111737
    • GTX111737 IHC-P Image
      Adenylate kinase 2 antibody [N1C3-2] detects Adenylate kinase 2 protein at mitochondria in rat ovary by immunohistochemical analysis.
      Sample: Paraffin-embedded rat ovary.
      Adenylate kinase 2 antibody [N1C3-2] (GTX111737) diluted at 1:500.
      Top
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX111737
    • GTX111737 IHC-P Image
      Adenylate kinase 2 antibody [N1C3-2] detects Adenylate kinase 2 protein at mitochondria in rat pancreas by immunohistochemical analysis.
      Sample: Paraffin-embedded rat pancreas.
      Adenylate kinase 2 antibody [N1C3-2] (GTX111737) diluted at 1:500.
      Top
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX111737
    • GTX111737 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Adenylate kinase 2(GTX111737) antibody at 1:500 dilution.
      Top
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in Western Blot (WB). GTX111737
    • GTX111737 WB Image
      Sample (50 ug of whole cell lysate)
      A: mouse brain
      12% SDS PAGE
      GTX111737 diluted at 1:1000
      Top
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111737
    • GTX111737 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AK2(GTX111737) antibody at 1:200 dilution.
      Top
  • Anti-Adenylate kinase 2 antibody [N1C3-2] used in Western Blot (WB). GTX111737
    • GTX111737 WB Image
      Sample (30 ug of whole cell lysate)
      A: Molt-4 (GTX27912)
      12% SDS PAGE
      GTX111737 diluted at 1:1000
      Top
Back to Top
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensAK2204P54819103020, 267500NP_00130606820.78
Homo sapiensAK2204P54819103020, 267500NP_00130606921.64
Homo sapiensAK2204P54819103020, 267500NP_00130607025.63
Homo sapiensAK2204P54819103020, 267500NP_00130607121.23
Homo sapiensAK2204P54819103020, 267500NM_001199199NP_00118612824.65
Homo sapiensAK2204P54819103020, 267500NM_001625NP_00161626.48
Homo sapiensAK2204P54819103020, 267500NM_013411NP_03754325.62

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusAk211637Q9WTP6NM_016895NP_05859125.61
Rattus norvegicusAk224184P29410NM_001033967NP_00102913925.53
Mus musculusAk211637Q9WTP6NM_001033966NP_00102913826.47
Rattus norvegicusAk224184P29410NM_030986NP_11224826.4
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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