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1 .GTX101902 Annexin II antibody

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Annexin II antibody

Knockout/Knockdown
Protein Overexpression

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman, Mouse, Rat
  • Publication  ( 5 )
Cat No: GTX101902
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-Annexin II antibody used in Western Blot (WB). GTX101902
  • Anti-Annexin II antibody used in Western Blot (WB). GTX101902
  • Anti-Annexin II antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX101902
  • Anti-Annexin II antibody used in Western Blot (WB). GTX101902
  • Anti-Annexin II antibody used in Western Blot (WB). GTX101902
  • Anti-Annexin II antibody used in Western Blot (WB). GTX101902
  • Anti-Annexin II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101902
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    Reference (5)
  • Bio-info
  • Protocol & MSDS
  • Application Information: Annexin II antibody

Catalog Number GTX101902
☆☆☆☆☆
( 0 )
Publication ( 5 )
Product Name Annexin II antibody
See all Annexin II products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000* Y
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size39 kDa (note) IsoForm (4)
Positive ControlsA549 , HCT116 , HeLa , GL261 , NIH-3T3 , C2C12 , Rat2 , human ANXA2-transfected 293T
Form SuppliedLiquid
Concentration0.62 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Annexin II antibody

Full Nameannexin A2
BackgroundThis gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
SynonymsANX2 antibody, ANX2L4 antibody, CAL1H antibody, LIP2 antibody, LPC2 antibody, LPC2D antibody, P36 antibody, PAP-IV antibody, ANXA2 antibody, placental anticoagulant protein IV antibody, calpactin I heavy polypeptide antibody, annexin II antibody, lipocortin II antibody, calpactin-1 heavy chain antibody, chromobindin 8 antibody, chromobindin-8 antibody, annexin-2 antibody, annexin A2 antibody, calpactin I heavy chain antibody, protein I antibody
Cellular LocalizationSecreted , extracellular space , extracellular matrix , basement membrane , Melanosome
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human Annexin II. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Xenopus laevis, Dog, Pig, Chicken, Sheep, Bovine
Predict Reactivity NoteXenopus laevis (81%), Dog (100%), Pig (100%), Chicken (90%), Sheep (99%), Bovine (99%)
ConjugationUnconjugated

  • Storage Conditions: Annexin II antibody

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Angiogenesis
  • Disease Related > Cardiovascular > Angiogenesis
  • Disease Related > Cardiovascular > Blood > Erythrocyte
  • Stem Cell Development > Development > Mesoderm development > Skeletal system development
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Annexin II antibody validated data
  • Anti-Annexin II antibody used in Western Blot (WB). GTX101902
    • GTX101902 WB Image  Knockout/Knockdown
      Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody (GTX101902) diluted at 1:1000.
      CRISPR knockout cell lines generated by www.edigene.com
      Top
  • Anti-Annexin II antibody used in Western Blot (WB). GTX101902
    • GTX101902 WB Image  Protein Overexpression
      Annexin II antibody detects Annexin II protein by western blot analysis. Non-transfected (-) and human Annexin I or Annexin II-transfected (+) whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody (GTX101902) diluted at 1:1000.
      Top
  • Anti-Annexin II antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX101902
    • GTX101902 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Annexin II antibody(GTX101902) antibody at 1:500 dilution.
      Top
  • Anti-Annexin II antibody used in Western Blot (WB). GTX101902
    • GTX101902 WB Image
      Annexin II antibody detects ANXA2 protein by Western blot analysis.
      A. 30 µg A549 whole cell lysate/extract
      B. 30 µg HCT116 whole cell lysate/extract
      10 % SDS-PAGE
      Annexin II antibody (GTX101902) dilution: 1:1000
      Top
  • Anti-Annexin II antibody used in Western Blot (WB). GTX101902
    • GTX101902 WB Image
      Annexin II antibody detects ANXA2 protein by Western blot analysis.
      A. 30 µg GL261 whole cell lysate/extract
      B. 30 µg NIH-3T3 whole cell lysate/extract
      C. 30 µg C2C12 whole cell lysate/extract
      10 % SDS-PAGE
      Annexin II antibody (GTX101902) dilution: 1:1000
      Top
  • Anti-Annexin II antibody used in Western Blot (WB). GTX101902
    • GTX101902 WB Image
      Annexin II antibody detects ANXA2 protein by Western blot analysis.
      A. 30 µg Rat2 whole cell lysate/extract
      10 % SDS-PAGE
      Annexin II antibody (GTX101902) dilution: 1:1000
      Top
  • Anti-Annexin II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101902
    • GTX101902 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed A431, using Annexin II (GTX101902) antibody at 1:200 dilution.
      Top
  • Anti-Annexin II antibody used in Western Blot (WB). GTX101902
    • GTX101902 WB Image  Knockout/Knockdown
      Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody (GTX101902) diluted at 1:1000.
      CRISPR knockout cell lines generated by www.edigene.com
      Top
  • Anti-Annexin II antibody used in Western Blot (WB). GTX101902
    • GTX101902 WB Image  Protein Overexpression
      Annexin II antibody detects Annexin II protein by western blot analysis. Non-transfected (-) and human Annexin I or Annexin II-transfected (+) whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody (GTX101902) diluted at 1:1000.
      Top
  • Anti-Annexin II antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX101902
    • GTX101902 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Annexin II antibody(GTX101902) antibody at 1:500 dilution.
      Top
  • Anti-Annexin II antibody used in Western Blot (WB). GTX101902
    • GTX101902 WB Image
      Annexin II antibody detects ANXA2 protein by Western blot analysis.
      A. 30 µg A549 whole cell lysate/extract
      B. 30 µg HCT116 whole cell lysate/extract
      10 % SDS-PAGE
      Annexin II antibody (GTX101902) dilution: 1:1000
      Top
  • Anti-Annexin II antibody used in Western Blot (WB). GTX101902
    • GTX101902 WB Image
      Annexin II antibody detects ANXA2 protein by Western blot analysis.
      A. 30 µg GL261 whole cell lysate/extract
      B. 30 µg NIH-3T3 whole cell lysate/extract
      C. 30 µg C2C12 whole cell lysate/extract
      10 % SDS-PAGE
      Annexin II antibody (GTX101902) dilution: 1:1000
      Top
  • Anti-Annexin II antibody used in Western Blot (WB). GTX101902
    • GTX101902 WB Image
      Annexin II antibody detects ANXA2 protein by Western blot analysis.
      A. 30 µg Rat2 whole cell lysate/extract
      10 % SDS-PAGE
      Annexin II antibody (GTX101902) dilution: 1:1000
      Top
  • Anti-Annexin II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101902
    • GTX101902 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed A431, using Annexin II (GTX101902) antibody at 1:200 dilution.
      Top
Back to Top
  • Reference for Annexin II antibody
Application References Application Reactivity
Kowal J et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A 113 (8):E968-77 (2016). WB Human
Gangalum RK et al. Inhibition of the Expression of the Small Heat Shock Protein αB-crystallin Inhibits Exosome Secretion in Human Retinal Pigment Epithelial Cells in Culture. J Biol Chem (Epub ahead of print) (2016). WB Human
Chao PZ et al. Dendritic cells respond to nasopharygeal carcinoma cells through annexin A2-recognizing DC-SIGN. Oncotarget 6 (1):159-70 (2015). Mouse
Babina IS et al. A novel mechanism of regulating breast cancer cell migration via palmitoylation-dependent alterations in the lipid raft affiliation of CD44. Breast Cancer Res 16 (1):R19 (2014). WB Human
Bulloj A et al. PI 3-kinase independent role for AKT in F-actin regulation during outer segment phagocytosis by RPE cells. Exp Eye Res 113:9-18 (2013). ICC/IF Rat
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensANXA2302P07355, Q6N0B3151740NM_001002857NP_00100285738.61
Homo sapiensANXA2302P07355, Q6N0B3151740NM_001002858NP_00100285840.42
Homo sapiensANXA2302P07355, Q6N0B3151740NM_001136015NP_00112948738.61
Homo sapiensANXA2302P07355, Q6N0B3151740NM_004039NP_00403038.61

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusAnxa212306P07356, Q542G9NM_007585NP_03161138.68
Rattus norvegicusAnxa256611Q07936NM_019905NP_06397038.68
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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