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Annexin II antibody [N1], N-term

KO/KD Overexpression
Anti-Annexin II antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100046
Anti-Annexin II antibody [N1], N-term used in Western Blot (WB). GTX100046
Anti-Annexin II antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100046
Anti-Annexin II antibody [N1], N-term used in Western Blot (WB). GTX100046
Anti-Annexin II antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100046
Anti-Annexin II antibody [N1], N-term used in Western Blot (WB). GTX100046

Cat. No. GTX100046

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Annexin II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

A549 , HCT116 , human ANXA2-transfected 293T , HeLa

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Dog, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Annexin II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240455

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

annexin A2 , ANX2 , ANX2L4 , CAL1H , HEL-S-270 , LIP2 , LPC2 , LPC2D , P36 , PAP-IV

Cellular Localization

Secreted , extracellular space , extracellular matrix , basement membrane , Melanosome

Background

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Annexin II antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100046

GTX100046 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Annexin II(GTX100046) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Annexin II antibody [N1], N-term used in Western Blot (WB). GTX100046

GTX100046 WB Image

KO/KD

Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody [N1], N-term (GTX100046) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin II antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100046

GTX100046 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue, using ANXA2(GTX100046) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Annexin II antibody [N1], N-term used in Western Blot (WB). GTX100046

GTX100046 WB Image

Overexpression

Annexin II antibody detects Annexin II protein by western blot analysis. Non-transfected (-) and human Annexin I or Annexin II-transfected (+) whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody (GTX100046) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin II antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100046

GTX100046 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue, using ANXA2(GTX100046) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Annexin II antibody [N1], N-term used in Western Blot (WB). GTX100046

GTX100046 WB Image

Annexin II antibody [N1], N-term detects ANXA2 protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg HCT116 whole cell lysate/extract
10% SDS-PAGE
Annexin II antibody [N1], N-term (GTX100046) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169