|Background||The Annexin V-PE Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation. |
‧ Detection method- Flow cytometry (Ex = 488 nm; Em = 578 nm) and fluorescence microscopy
‧ Sample type- Live cells
‧ Species reactivity- Mammalian
‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Features and Benefits
‧ Simple one-step procedure; takes only 10 minutes
‧ Fast and convenient, no need for fixation of cells
‧ The Annexin V-PE Apoptosis Detection Kit contains the bright orange-red PE fluorescent probe that can be easily detected by flow cytometry or fluorescence microscopy.
1X Binding Buffer