Arginase 1 antibody

Cat. No. GTX113131

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA, IHC, Sandwich ELISA
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Arginase 1 products

View product citations for antibody GTX113131 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
ELISA	1:1000-1:10000
IHC	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX634218 / GTX113131, Detection : GTX113131 / GTX634218

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

human liver

Predict Reactivity

Rabbit, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Arginase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036265

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

arginase 1

Cellular Localization

Cytoplasm

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]

Database

Gene ID: 383 ARG1
UniProt: P05089 ARG1

Research Area

DATA IMAGES

GTX113131 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX113131 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX113131 WB Image

Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX113131) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113131 IHC-P Image

Arginase 1 antibody detects ARG1 protein at cytosol on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
Arginase 1 antibody (GTX113131) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113131 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX113131) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113131 ELISA Image

Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX113131 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX113131 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX113131 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.