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  • Aspartoacylase antibody [N1C3-2]

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1 .GTX113389 Aspartoacylase antibody [N1C3-2]

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Aspartoacylase antibody [N1C3-2]


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman, Mouse
  • Publication  ( 3 )
Cat No: GTX113389
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-Aspartoacylase antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113389
  • Anti-Aspartoacylase antibody [N1C3-2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX113389
  • Anti-Aspartoacylase antibody [N1C3-2] used in Western Blot (WB). GTX113389
  • Anti-Aspartoacylase antibody [N1C3-2] used in Western Blot (WB). GTX113389
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  • Application Information: Aspartoacylase antibody [N1C3-2]

Catalog Number GTX113389
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Publication ( 3 )
Product Name Aspartoacylase antibody [N1C3-2]
See all Aspartoacylase products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size36 kDa (note) IsoForm (3)
Positive ControlsMolt-4 , Raji , U87-MG , SK-N-SH , IMR32 , SK-N-AS
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Aspartoacylase antibody [N1C3-2]

Full Nameaspartoacylase
BackgroundThis gene encodes an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues. Mutations in this gene cause Canavan disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]
SynonymsACY2 antibody, ASP antibody, ASPA antibody, aspartoacylase antibody, aminoacylase-2 antibody, aminoacylase 2 antibody, ACY-2 antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human Aspartoacylase. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Pig, Rhesus Monkey, Bovine
Predict Reactivity NoteRat (85%), Pig (95%), Rhesus Monkey (97%), Bovine (93%)
ConjugationUnconjugated

  • Storage Conditions: Aspartoacylase antibody [N1C3-2]

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Aspartoacylase antibody [N1C3-2] validated data
  • Anti-Aspartoacylase antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113389
    • GTX113389 ICC/IF Image
      Aspartoacylase antibody [N1C3-2] detects Aspartoacylase protein at cytoplasm by immunofluorescent analysis.
      Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: Aspartoacylase protein stained by Aspartoacylase antibody [N1C3-2] (GTX113389) diluted at 1:500.
      Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Aspartoacylase antibody [N1C3-2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX113389
    • GTX113389 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Aspartoacylase(GTX113389) antibody at 1:100 dilution.
      Top
  • Anti-Aspartoacylase antibody [N1C3-2] used in Western Blot (WB). GTX113389
    • GTX113389 WB Image
      Sample (30 µg of whole cell lysate)
      A: Molt-4 (GTX27912)
      B: Raji
      10% SDS PAGE
      GTX113389 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Aspartoacylase antibody [N1C3-2] used in Western Blot (WB). GTX113389
    • GTX113389 WB Image
      Aspartoacylase antibody detects Aspartoacylase protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aspartoacylase antibody (GTX113389) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Aspartoacylase antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113389
    • GTX113389 ICC/IF Image
      Aspartoacylase antibody [N1C3-2] detects Aspartoacylase protein at cytoplasm by immunofluorescent analysis.
      Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: Aspartoacylase protein stained by Aspartoacylase antibody [N1C3-2] (GTX113389) diluted at 1:500.
      Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Aspartoacylase antibody [N1C3-2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX113389
    • GTX113389 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Aspartoacylase(GTX113389) antibody at 1:100 dilution.
      Top
  • Anti-Aspartoacylase antibody [N1C3-2] used in Western Blot (WB). GTX113389
    • GTX113389 WB Image
      Sample (30 µg of whole cell lysate)
      A: Molt-4 (GTX27912)
      B: Raji
      10% SDS PAGE
      GTX113389 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Aspartoacylase antibody [N1C3-2] used in Western Blot (WB). GTX113389
    • GTX113389 WB Image
      Aspartoacylase antibody detects Aspartoacylase protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aspartoacylase antibody (GTX113389) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
Back to Top
  • Reference for Aspartoacylase antibody [N1C3-2]
Application References Application Reactivity
Gautier EL et al. Gata6 regulates aspartoacylase expression in resident peritoneal macrophages and controls their survival. J Exp Med 211 (8):1525-31 (2014). WB Mouse
Long PM et al. N-acetylaspartate (NAA) and N-acetylaspartylglutamate (NAAG) promote growth and inhibit differentiation of glioma stem-like cells. J Biol Chem 288 (36):26188-200 (2013). WB, ICC/IF Mouse
Sommer A et al. Expression of aspartoacylase (ASPA) and Canavan disease. Gene 505 (2):206-10 (2012). WB Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensASPA443P45381, Q6FH48271900, 608034BC029128AAH2912835.74
Homo sapiensASPA443P45381, Q6FH48271900, 608034NM_000049NP_00004035.74
Homo sapiensASPA443P45381, Q6FH48271900, 608034NM_001128085NP_00112155735.74

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusAspa11484Q8R3P0NM_023113NP_07560235.35
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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