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  • Asporin antibody [N3C1], Internal

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1 .GTX104790 Asporin antibody [N3C1], Internal

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Asporin antibody [N3C1], Internal


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, WB
  • Species reactivityHuman, Mouse, Rat
  • Publication  ( 1 )
Cat No: GTX104790
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790
  • Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790
  • Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790
  • Anti-Asporin antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104790
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  • Application Information: Asporin antibody [N3C1], Internal

Catalog Number GTX104790
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Publication ( 1 )
Product Name Asporin antibody [N3C1], Internal
See all Asporin products
ApplicationsICC/IF, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size43 kDa (note) IsoForm (2)
Positive ControlsA549 , H1299 , HCT116 , mouse liver , rat heart
Form SuppliedLiquid
Concentration0.43 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Asporin antibody [N3C1], Internal

Full Nameasporin
BackgroundASPN belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin (MIM 125255) (Lorenzo et al., 2001 [PubMed 11152692]).[supplied by OMIM]
SynonymsFLJ20129 antibody, OS3 antibody, PLAP-1 antibody, PLAP1 antibody, SLRR1C antibody, ASPN antibody, asporin antibody, asporin (LRR class 1) antibody, small leucine-rich protein 1C antibody, asporin proteoglycan antibody, periodontal ligament associated protein 1 antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenCarrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Asporin. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
 		Pig, Bovine
Predict Reactivity NotePig (92%), Bovine (85%)
ConjugationUnconjugated

  • Storage Conditions: Asporin antibody [N3C1], Internal

Storage Buffer1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionStore as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Immunology > Autoimmunity > Autoimmune diseases > Rheumatoid Arthritis
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Asporin antibody [N3C1], Internal validated data
  • Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790
    • GTX104790 WB Image
      Asporin antibody [N3C1], Internal detects Asporin protein by western blot analysis.
      A. 30 μg A549 whole cell lysate/extract
      B. 30 μg H1299 whole cell lysate/extract
      C. 30 μg HCT116 whole cell lysate/extract
      10 % SDS-PAGE
      Asporin antibody [N3C1], Internal (GTX104790) dilution: 1:1000
      Top
  • Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790
    • GTX104790 WB Image
      Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal (GTX104790) diluted at 1:500.
      Top
  • Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790
    • GTX104790 WB Image
      Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal (GTX104790) diluted at 1:500.
      Top
  • Anti-Asporin antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104790
    • GTX104790 ICC/IF Image
      Immunofluorescence analysis of methanol-fixed HeLa, using Asporin(GTX104790) antibody at 1:200 dilution.
      Top
  • Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790
    • GTX104790 WB Image
      Asporin antibody [N3C1], Internal detects Asporin protein by western blot analysis.
      A. 30 μg A549 whole cell lysate/extract
      B. 30 μg H1299 whole cell lysate/extract
      C. 30 μg HCT116 whole cell lysate/extract
      10 % SDS-PAGE
      Asporin antibody [N3C1], Internal (GTX104790) dilution: 1:1000
      Top
  • Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790
    • GTX104790 WB Image
      Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal (GTX104790) diluted at 1:500.
      Top
  • Anti-Asporin antibody [N3C1], Internal used in Western Blot (WB). GTX104790
    • GTX104790 WB Image
      Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal (GTX104790) diluted at 1:500.
      Top
  • Anti-Asporin antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104790
    • GTX104790 ICC/IF Image
      Immunofluorescence analysis of methanol-fixed HeLa, using Asporin(GTX104790) antibody at 1:200 dilution.
      Top
Back to Top
  • Reference for Asporin antibody [N3C1], Internal
Application References Application Reactivity
Rochette A et al. Asporin is a stromally expressed marker associated with prostate cancer progression. Br J Cancer 116 (6):775-784 (2017).
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensASPN54829Q9BXN1603932, 607850, 608135NM_001193335NP_00118026427.64
Homo sapiensASPN54829Q9BXN1603932, 607850, 608135NM_017680NP_06015043.42
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-Thimerosal.pdf

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