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BACE2 antibody [C1C3]

Anti-BACE2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113951
Anti-BACE2 antibody [C1C3] used in Western Blot (WB). GTX113951
Anti-BACE2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX113951
Anti-BACE2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX113951
Anti-BACE2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX113951

Cat. No. GTX113951

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all BACE2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BACE2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10726135

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

beta-secretase 2 , AEPLC , ALP56 , ASP1 , ASP21 , BAE2 , CDA13 , CEAP1 , DRAP

Cellular Localization

Membrane

Background

Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease and a frequent complication of Down syndrome. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein by 2 proteases, one of which is the protein encoded by this gene. This gene localizes to the 'Down critical region' of chromosome 21. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease. Three transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BACE2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113951

GTX113951 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using BACE2(GTX113951) antibody at 1:500 dilution.

Anti-BACE2 antibody [C1C3] used in Western Blot (WB). GTX113951

GTX113951 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
GTX113951 diluted at 1:1000

Anti-BACE2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX113951

GTX113951 IHC-P Image

BACE2 antibody [C1C3] detects BACE2 protein at cytoplasm in Rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded Rat brain.
BACE2 antibody [C1C3] (GTX113951) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BACE2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX113951

GTX113951 IHC-P Image

BACE2 antibody [C1C3] detects BACE2 protein at cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
BACE2 antibody [C1C3] (GTX113951) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BACE2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX113951

GTX113951 IHC-P Image

BACE2 antibody [C1C3] detects BACE2 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
BACE2 antibody [C1C3] (GTX113951) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Ren J et al. Acta Biochim Biophys Sin (Shanghai) 2022; 54 (1) : 77-90 MEF2C ameliorates learning, memory, and molecular pathological changes in Alzheimer’s disease in vivo and in vitro.
Application : WB
Reactivity : Mouse
Zhao L et al. Am J Pathol 2018; (Epub) Protection against the Neurotoxic Effects of β-Amyloid Peptide on Cultured Neuronal Cells by * Involves Elevated Expression of α7 Nicotinic Acetylcholine Receptors and Activating Phosphorylation of Protein Kinases.
Application : WB
Reactivity : Rat
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169