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BAG2 antibody [N1C3-2]

KO/KD
Anti-BAG2 antibody [N1C3-2] used in Western Blot (WB). GTX117345
Anti-BAG2 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX117345
Anti-BAG2 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117345
Anti-BAG2 antibody [N1C3-2] used in Western Blot (WB). GTX117345

Cat. No. GTX117345

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all BAG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

A549 , HeLa

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BAG2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729208

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 associated athanogene 2 , BAG-2 , dJ417I1.2

Background

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BAG2 antibody [N1C3-2] used in Western Blot (WB). GTX117345

GTX117345 WB Image

KO/KD

Wild-type (WT) and BAG2 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BAG2 antibody [N1C3-2] (GTX117345) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BAG2 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX117345

GTX117345 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using BAG2(GTX117345) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BAG2 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117345

GTX117345 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using BAG2(GTX117345) antibody at 1:200 dilution.

Anti-BAG2 antibody [N1C3-2] used in Western Blot (WB). GTX117345

GTX117345 WB Image

Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
GTX117345 diluted at 1:5000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169