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BCKDHA antibody [C1C3]

Anti-BCKDHA antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111382
Anti-BCKDHA antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111382
Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382
Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382
Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382

Cat. No. GTX111382

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Sheep, Bovine, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BCKDHA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177837

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

branched chain keto acid dehydrogenase E1, alpha polypeptide , BCKDE1A , MSU , MSUD1 , OVD1A

Cellular Localization

Mitochondrion matrix

Background

The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine. This reaction is the second major step in the catabolism of the branched-chain amino acids (Wynn et al., 1998 [PubMed 9582350]). The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2; MIM 248610), and a homodimeric dihydrolipoamide dehydrogenase (E3; MIM 238331). E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene (MIM 248611) encodes the beta subunit of E1. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-BCKDHA antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111382

GTX111382 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using BCKDHA(GTX111382) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BCKDHA antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111382

GTX111382 ICC/IF Image

BCKDHA antibody [C1C3] detects BCKDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: BCKDHA protein stained by BCKDHA antibody [C1C3] (GTX111382) diluted at 1:400.
Blue: Hoechst 33342 staining.

Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382

GTX111382 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX111382 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382

GTX111382 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [C1C3] (GTX111382) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCKDHA antibody [C1C3] used in Western Blot (WB). GTX111382

GTX111382 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [C1C3] (GTX111382) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169