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1 .GTX112744 BCL10 antibody

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BCL10 antibody

Knockout/Knockdown

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, IP, WB
  • Species reactivityHuman, Mouse
Cat No: GTX112744
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-BCL10 antibody used in Western Blot (WB). GTX112744
  • Anti-BCL10 antibody used in Immunoprecipitation. GTX112744
  • Anti-BCL10 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX112744
  • Anti-BCL10 antibody used in Western Blot (WB). GTX112744
  • Anti-BCL10 antibody used in Western Blot (WB). GTX112744
  • Anti-BCL10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112744
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  • Application Information: BCL10 antibody

Catalog Number GTX112744
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Product Name BCL10 antibody
See all BCL10 products
ApplicationsICC/IF, IHC-P, IP, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Immunoprecipitation1:100-1:500*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size26 kDa (note) IsoForm (2)
Positive ControlsHeLa , Raji , Neuro2A , GL261 , C8D30
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: BCL10 antibody

Full NameB-cell CLL/lymphoma 10
BackgroundThis gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. [provided by RefSeq]
SynonymsCARMEN antibody, CIPER antibody, CLAP antibody, c-E10 antibody, mE10 antibody, BCL10 antibody, B-cell lymphoma/leukemia 10 antibody, cellular-E10 antibody, cCARMEN antibody, CARD containing molecule enhancing NF-kB antibody, hCLAP antibody, CED-3/ICH-1 prodomain homologous E10-like regulator antibody, mammalian CARD-containing adapter molecule E10 antibody, CARD-containing apoptotic signaling protein antibody, CARD-containing proapoptotic protein antibody, caspase-recruiting domain-containing protein antibody, cellular homolog of vCARMEN antibody, CARD-containing molecule enhancing NF-kappa-B antibody, B-cell CLL/lymphoma 10 antibody
Cellular LocalizationCytoplasm , perinuclear region
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human BCL10. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Pig, Rhesus Monkey, Bovine
Predict Reactivity NoteRat (92%), Pig (92%), Rhesus Monkey (99%), Bovine (95%)
ConjugationUnconjugated

  • Storage Conditions: BCL10 antibody

Storage Buffer1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Type of cancer > Germ Cell and Embryonal
  • Cancer > Type of cancer > Testicular
  • Cell Biology > Apoptosis > Inflammatory cell > B cell
  • Immunology > Innate immune response
More Hide
BCL10 antibody validated data
  • Anti-BCL10 antibody used in Western Blot (WB). GTX112744
    • GTX112744 WB Image
      Wild-type (WT) and BCL10 knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL10 antibody (GTX112744) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      CRISPR knockout cell lines generated by www.edigene.com
      Top
  • Anti-BCL10 antibody used in Immunoprecipitation. GTX112744
    • GTX112744 IP Image
      BCL10 antibody immunoprecipitates BCL10 protein in IP experiments.
      IP samples: Raji whole cell extract
      A. 40 μg Raji whole cell extract
      B. Control with 4 μg of preimmune Rabbit IgG
      C. Immunoprecipitation of BCL10 protein by 4 μg BCL10 antibody (GTX112744)
      12 % SDS-PAGE
      The immunoprecipitated BCL10 protein was detected by BCL10 antibody (GTX112744) diluted at 1:500.
      [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
      Top
  • Anti-BCL10 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX112744
    • GTX112744 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human gastric cancer, using BCL10 antibody(GTX112744) antibody at 1:500 dilution.
      Top
  • Anti-BCL10 antibody used in Western Blot (WB). GTX112744
    • GTX112744 WB Image
      Sample (30 ug of whole cell lysate)
      A: Raji
      12% SDS PAGE
      GTX112744 diluted at 1:1000
      Top
  • Anti-BCL10 antibody used in Western Blot (WB). GTX112744
    • GTX112744 WB Image
      BCL10 antibody detects BCL10 protein by Western blot analysis.
      A. 30 µg Neuro2A whole cell lysate/extract
      B. 30 µg GL261 whole cell lysate/extract
      C. 30 µg C8D30 whole cell lysate/extract
      12 % SDS-PAGE
      BCL10 antibody (GTX112744) dilution: 1:1000
      Top
  • Anti-BCL10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112744
    • GTX112744 ICC/IF Image
      BCL10 antibody detects BCL10 protein at cytoplasm by immunofluorescent analysis.
      Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
      Green: BCL10 protein stained by BCL10 antibody (GTX112744) diluted at 1:500.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-BCL10 antibody used in Western Blot (WB). GTX112744
    • GTX112744 WB Image
      Wild-type (WT) and BCL10 knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL10 antibody (GTX112744) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      CRISPR knockout cell lines generated by www.edigene.com
      Top
  • Anti-BCL10 antibody used in Immunoprecipitation. GTX112744
    • GTX112744 IP Image
      BCL10 antibody immunoprecipitates BCL10 protein in IP experiments.
      IP samples: Raji whole cell extract
      A. 40 μg Raji whole cell extract
      B. Control with 4 μg of preimmune Rabbit IgG
      C. Immunoprecipitation of BCL10 protein by 4 μg BCL10 antibody (GTX112744)
      12 % SDS-PAGE
      The immunoprecipitated BCL10 protein was detected by BCL10 antibody (GTX112744) diluted at 1:500.
      [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
      Top
  • Anti-BCL10 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX112744
    • GTX112744 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human gastric cancer, using BCL10 antibody(GTX112744) antibody at 1:500 dilution.
      Top
  • Anti-BCL10 antibody used in Western Blot (WB). GTX112744
    • GTX112744 WB Image
      Sample (30 ug of whole cell lysate)
      A: Raji
      12% SDS PAGE
      GTX112744 diluted at 1:1000
      Top
  • Anti-BCL10 antibody used in Western Blot (WB). GTX112744
    • GTX112744 WB Image
      BCL10 antibody detects BCL10 protein by Western blot analysis.
      A. 30 µg Neuro2A whole cell lysate/extract
      B. 30 µg GL261 whole cell lysate/extract
      C. 30 µg C8D30 whole cell lysate/extract
      12 % SDS-PAGE
      BCL10 antibody (GTX112744) dilution: 1:1000
      Top
  • Anti-BCL10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112744
    • GTX112744 ICC/IF Image
      BCL10 antibody detects BCL10 protein at cytoplasm by immunofluorescent analysis.
      Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
      Green: BCL10 protein stained by BCL10 antibody (GTX112744) diluted at 1:500.
      Blue: Hoechst 33342 staining.
      Top
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Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensBCL108915O95999, Q5VUF1603517NP_00130764425.1
Homo sapiensBCL108915O95999, Q5VUF1603517NM_003921NP_00391226.25

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusBcl1012042Q9Z0H7NM_009740NP_03387025.95
Rattus norvegicusBcl1083477Q9QYN5NM_031328NP_11261826
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-Thimerosal.pdf
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All products are for research use only—Not for use in diagnostic procedures.