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BCL6 antibody [N2C1], Internal

Anti-BCL6 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX101338
Anti-BCL6 antibody [N2C1], Internal used in Western Blot (WB). GTX101338
Anti-BCL6 antibody [N2C1], Internal used in Western Blot (WB). GTX101338
Anti-BCL6 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX101338
Anti-BCL6 antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX101338
Anti-BCL6 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX101338
Anti-BCL6 antibody [N2C1], Internal used in Western Blot (WB). GTX101338

Cat. No. GTX101338

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Dog
Package
100 μl ($399),
25 μl ($169)
See all BCL6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BCL6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617798

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL6 transcription repressor , BCL5 , BCL6A , LAZ3 , ZBTB27 , ZNF51

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BCL6 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX101338

GTX101338 IHC-Fr Image

BCL6 antibody [N2C1], Internal detects BCL6 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: BCL6 stained by BCL6 antibody [N2C1], Internal (GTX101338) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-BCL6 antibody [N2C1], Internal used in Western Blot (WB). GTX101338

GTX101338 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BCL6 antibody (GTX101338) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-BCL6 antibody [N2C1], Internal used in Western Blot (WB). GTX101338

GTX101338 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with BCL6 antibody [N2C1], Internal (GTX101338) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCL6 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX101338

GTX101338 IP Image

BCL6 antibody immunoprecipitates BCL6 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 μg whole cell lysate/extract of BCL6 protein expressing Raji cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of BCL6 by 3 μg of BCL6 antibody (GTX101338) 7.5% SDS-PAGE The immunoprecipitated BCL6 protein was detected by BCL6 antibody (GTX101338) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-BCL6 antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX101338

GTX101338 ChIP assay Image

Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-BCL6 antibody. The precipitated DNA was detected by PCR with primer set targeting to PRDM1 or CD20.
ChIP experiment and primer designs are based on Blood. 2007 Sep 15;110(6):2067-74.

Anti-BCL6 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX101338

GTX101338 IHC-P Image

BCL6 antibody [N2C1], Internal detects BCL6 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
BCL6 stained by BCL6 antibody [N2C1], Internal (GTX101338) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BCL6 antibody [N2C1], Internal used in Western Blot (WB). GTX101338

GTX101338 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BCL6 antibody [N2C1], Internal (GTX101338) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Liu J et al. World Allergy Organ J 2023; 16 (8) : 100807 Changes of allergic inflammation and immunological parameters after Alt a 1 and A.?alternata immunotherapy in mice.
Juan Liu et al. World Allergy Organ. J. 2023; 16 (8) : 100807 Changes of allergic inflammation and immunological parameters after Alt a 1 and A. alternata immunotherapy in mice
Application : IHC-P
Reactivity : Mouse
Chen D et al. Cell Death Dis 2017; 8 (10) : e3156 BCL6 attenuates renal inflammation via negative regulation of NLRP3 transcription.
Reactivity : Rat
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169