| Catalog Number | GTX70111 |
| Product Name | BRCA1 antibody [17F8] - ChIP grade
see all 'BRCA1' products |
| Full Name | breast cancer 1, early onset |
| Synonyms | | 113705 | RNF53 | BRCA1 | BRCAI | 672 | PSCP | BRCC1 | P38398 | IRIS | BROVCA1 | PNCA4 | |
| Product Description | Mouse monoclonal [17F8] to BRCA1 |
| Background | BRCA1-17F8 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the Aexon 11 splice variant. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation. |
| Specificity | This antibody does not recognize the delta exon 11 splice variant of BRCA1. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No | 17F8 |
| Subclass | IgG1 |
| Immunogen | Protein fragment expressed in E. coli corresponding to amino acids 762-1315. |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse |
| Applications | ChIP assay, ELISA, ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For IF: Use at a concentration of 1 µg/ml. For WB: Use at 1-10 µg/ml. Predicted molecular weight: 208 kDa. For IHC-P: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher |
| Positive Controls | HBL 100 nuclear lysate and T47D whole cell |
| Target | BRCA1 |
| Predicted Target Size(KDa) | 220 (Note) |
| Cellular Localization | Nuclear/Cytoplasmic |
| Form Supplied | Liquid |
| Concentration | 1 mg/ml |
| Purification | Protein G purified |
| Purification Note | From mouse ascitic fluid |
| Storage Buffer | Phosphate-buffered saline, pH7.2 containing no preservatives |
| Storage Instruction | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |