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BRCA2 antibody [5F6]

Anti-BRCA2 antibody [5F6] used in Western Blot (WB). GTX70123

Cat. No. GTX70123

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5F6

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human
Package
100 μl ($399)
See all BRCA2 products

APPLICATION

Application Note

Do not boil samples for longer than two minutes. For WB: Use at a concentration of 1-5 μg/ml. Predicted molecular weight: 380 kDa. Optimal dilutions/concentrations should be determined by the reseracher.

Calculated MW

384 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 2336-2478 of human BRCA2 expressed in E. coli. BRCA2-P400.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_368624

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BRCA2 DNA repair associated , BRCC2 , BROVCA2 , FACD , FAD , FAD1 , FANCD , FANCD1 , GLM3 , PNCA2 , XRCC11

Cellular Localization

Nucleus

Background

Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-BRCA2 antibody [5F6] used in Western Blot (WB). GTX70123

GTX70123 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA2 antibody [5F6] (GTX70123) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Soni A et al. DNA Repair (Amst) 2021; 101 : 103076 Proficiency in homologous recombination repair is prerequisite for activation of G2-checkpoint at low radiation doses.
Bosviel R et al. OMICS 2012; 16 (5) : 235-44 Can soy phytoestrogens decrease DNA methylation in BRCA1 and BRCA2 oncosuppressor genes in breast cancer?
Application : IHC-P
Reactivity : Human
Bernard-Gallon DJ et al. Anticancer Res 2004; 24 (1) : 321-4 Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia.
Application : IHC-P
Reactivity : Human
Bernard-Gallon DJ et al. Breast Cancer Res 2001; 3 (1) : 22402 BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite.
Application : IHC-P
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations