|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||44 kDa (note)
|Positive Controls||A431 , HeLa , Neuro2A , GL261 , C8D30 , PC-12 , Rat2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||brain and reproductive organ-expressed (TNFRSF1A modulator)|
|Product Description||Rabbit Polyclonal antibody to BRCC45 (brain and reproductive organ-expressed (TNFRSF1A modulator))|
|Background||Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between MERIT40/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect. |
|Synonyms||BRCC4 antibody, BRCC45 antibody, BRE antibody, BRCA1/BRCA2-containing complex subunit 45 antibody, BRCA1/BRCA2-containing complex, subunit 4 antibody, BRCA1-A complex subunit BRE antibody, brain and reproductive organ-expressed protein antibody, brain and reproductive organ-expressed (TNFRSF1A modulator) antibody|
|Cellular Localization||Cytoplasm , Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human BRCC45. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Xenopus laevis, Chicken, Bovine|
|Predict Reactivity Note||Zebrafish (81%), Xenopus laevis (89%), Chicken (98%), Bovine (100%)|