Bax antibody [N1N2], N-term
|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||21 kDa (note)
|Positive Controls||293T , HeLaS3 , HepG2 , Molt-4 , Raji , *U2OS |
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||BCL2-associated X protein|
|Product Description||Rabbit polyclonal antibody to BAX (BCL2-associated X protein)|
|Background||The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq]|
|Synonyms||BCL2L4 antibody, BAX antibody, bcl-2-like protein 4 antibody, apoptosis regulator BAX antibody, bcl2-L-4 antibody, BCL2-associated X protein antibody|
|Cellular Localization||Isoform Alpha: Mitochondrion membrane , Cytoplasm , Isoform Beta: Cytoplasm , Isoform Gamma: Cytoplasm , Isoform Delta: Cytoplasm |
|Immunogen||Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bax. The exact sequence is proprietary.|
|Species Reactivity||Human, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Dog, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Dog (100%), Rhesus Monkey (100%), Bovine (100%)|