Bcl-2 antibody 
|Applications||FACS, IHC-Fr, IHC-P, IP, WB|
|IHC (Frozen sections)||1/20-1/40*
|IHC (Formalin-fixed paraffin-embedded sections)||1/20-1/40*
| Immunoprecipitation||Assay-dependent dilution
| Western blot||Assay-dependent dilution
Flow cytometry/FACS : Membrane permeabilisation is required for this application.Not tested in other applications.
IHC (Formalin-fixed paraffin-embedded sections) : This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein G purified|
|Purification Note||From tissue culture supernatant|
|Full Name||B-cell CLL/lymphoma 2|
|Product Description||Mouse Monoclonal antibody  to Bcl-2|
|Background||This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]|
|Synonyms||PPP1R50 Antibody , B-cell CLL/lymphoma 2 Antibody , BCL2 Antibody|
|Immunogen||Synthetic peptide, amino acids 41-54 of the Bcl-2 protein|